大穗黑麦草成熟种子愈伤组织诱导及分化的研究

以大穗黑麦草成熟种子为外植体 ,采用MS基本培养基 ,研究了不同浓度的 2 ,4-D和 6-BA及其组合对其愈伤组织诱导的影响 ,不同浓度的NAA和 6-BA组合对愈伤组织分化成苗的影响。结果表明 :不同浓度 2 ,4-D对大穗黑麦草愈伤组织诱导率差异显著 ,以 7mg/L效果最好 ,浓度过高 ,愈伤组织诱导率下降 ;在 0～ 1 .0mg/L范围内 ,6-BA浓度越高 ,愈伤组织诱导率越高 ,但长势渐差 ;2 ,4-D + 6-BA的不同配比浓度的愈伤组织诱导率存在差异 ,以MS + 7mg/L 2 ,4-D + 0mg/L 6-BA的组合为最优 ,产生的愈伤组织数量多、质量好 ;NAA+ 6-BA不同配比浓度的愈伤组织分化率存在一定差异 ,以MS +NAA 0 .5mg/L + 6-BA 1mg/L的愈伤组织分化率最高。初步建立了一个以大穗黑麦草成熟种子为外植体的愈伤组织诱导及分化体系

Studies on Callus Induction and Differentiation of Mature Seed of Lolium grandispicum Y·J·Fei

Taking the mature seed of Lolium grandspicum Y·J· Fei as explant and adopting MS basal medium,the influence on callus inducing by different concd.of 2,4-D,6-BA and their combination,as well as the influence on callus differentiation by NAA and 6-BA combination were studied.The results showed that different concd.2,4-D have significant variation on callus inducing ratio and the concd.of 7 mg/L is most effective.While with the increase of concd.the callus inducing ratio decrease.In 0-1.0 mg/L,the higher the 6-BA concd.is,the higher the callus inducing ratio is, but the weaker the growth is.Different concd.of 2,4-D+6-BA combination have variation on callus inducing and the combination of MS+7 mg/L 2,4-D+0 mg/L 6-BA is the optimum,which produce most callus with high quality.Different concd.of NAA+6-BA combination have variation on callus differentiation,with the highest differentiation on MS+NAA 0.5 mg/L+6-BA 1 mg /L.A callus inducing and differentiation system is established by taking the mature seed of Lolium grandispicum Y·J· Fei as explant.