Proteomic analysis of the hypothalamus of broiler‐type Taiwan country chickens in response to acute heat stress |
| |
| Authors: | Wei‐Lin Tu Chuen‐Yu Cheng Chao‐Jung Chen Hong‐Lin Chan Shih‐Han Wang Pin‐Chi Tang Chih‐Feng Chen Hsin‐Hsin Chen Yen‐Pai Lee Shuen‐Ei Chen San‐Yuan Huang |
| |
| Institution: | 1. Department of Animal Science, National Chung Hsing University, Taichung, Taiwan;2. Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan;3. Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan;4. Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan;5. Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan;6. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan;7. Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing University, Taichung, Taiwan;8. Research Center for Sustainable Energy and Nanotechnology, National Chung Hsing University, Taichung, Taiwan |
| |
| Abstract: | The hypothalamus is a critical center for regulating heat retention or dissipation. This study investigated global protein changes in the hypothalamus of broiler‐type Taiwan country chickens (TCCs) after acute heat stress. Twelve TCC hens aged 30 weeks were allocated to groups subjected to acute heat stress at 38°C for 2 hr without recovery, with 2 hr of recovery, and with 6 hr of recovery; a control group was maintained at 25°C. Hypothalami were collected for protein expression analysis at the end of each time point. The results showed 114 protein spots differentially expressed after acute heat stress. Most of the differentially expressed proteins were involved in cellular processes, metabolism, transport, and cellular component organization. Functional annotation analysis suggested that these proteins were related to cellular defensive responses against heat and oxidative stress, detoxification and toxin export/delivery, cytoskeleton integrity, oxygen transport, and neural development. The results of this study suggest that acute heat stress damages the hypothalamus of broiler‐type TCCs through oxidative stress and provokes a series of responses to stabilize protein structures, degrade misfolded proteins, and remodel cytoskeletons for attenuating the detrimental effects by acute heat stress. |
| |
| Keywords: | acute heat stress hypothalamus proteomics Taiwan country chickens |
|
|
