从大豆幼胚诱导胚胎发生再生植株

采用MS基本培养基附加高浓度的生长素(2,4—D或NAA)成功地诱导了大豆(G.max)未成熟胚的体细胞胚胎发生。2,4—D的诱导效果明显优于NAA。细胞分裂素对体细胞胚胎发生有抑制作用。适宜的维生素B1浓度为0.4ppm。体细胞胚胎发生频率随蔗糖浓度(1.5—9％)的提高而降低。体细胞胚胎经历诱导、成熟和发芽三个阶段成功地发育成完整植株。再生植株移入土壤已经获得种子。

PLANT REGENERATION FROM IMMATURE EMBRYO CULTURE OF SOYBEAN VIA SOMATIC EMBRYOGENESIS

Embryogeuesis from immature embryo of soybean (G. max) was induced succossfully by placing about 4 mm long embryos on Murashige and Skoog medium containing high concentration of 2, 4-dichlorophen-oxyacetic acid (2, 4-D) or a-naphthleneacetic acid (NAA). 2, 4-D was significantly superior to NAA in initiation effect. The best concentration of 2, 4-D was 20 ppm. In light, the highest embryogenesis frequency induced by 2, 4-D reached to 80.0%, the largest embryogenesis efficiency was 10.32, and 2.7 normal somatic embryos was obtained on an immatural cotyledon. Dark treatment reduced embryogenesis freque-ecey induced by 2,4-D. The embryogenesis was inhibited by cytokinin even at very low concentration. High concentration of vitamin B1 was unfavorable to the frequency of embryogenesis and it's best concentra tion was about 0.4 ppm. In an examination of the effects of sucrose concentration, the embryogenesis frequency decreased as sucrose concentration increased from 1.5 to 9%. The somatic embryo underwent successfully stages of inducing, maturing and germinating and (?)rew into coemplete plant. Seeds have been obtained by transplanting regenerated plants to soil.