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| 鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析 | |
| 引用本文： | 皇甫和平,王川庆,陈陆,杨霞,刘红英,彭志锋,郑鹿平,徐雪,刘慧敏,付仁一,郭伦涛. 鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析[J]. 农业科学与技术, 2009, 10(1): 43-46,55 |
| 作者姓名： | 皇甫和平 王川庆 陈陆 杨霞 刘红英 彭志锋 郑鹿平 徐雪 刘慧敏 付仁一 郭伦涛 |
| 作者单位： | 皇甫和平,HUANGFU He-ping(河南农业大学牧医工程学院,河南郑州,450002;郑州牧业工程高等专科学校,河南郑州,450011);王川庆,陈陆,杨霞,刘红英,彭志锋,郑鹿平,徐雪,刘慧敏,付仁一,郭伦涛,WANG Chuan-qing,CHEN Lu,YANG Xia,LIU Hong-ying,PENG Zhi-feng,ZHENG Lu-ping,XU Xue,LIU Hui-min,FU Ren-yi,GUO Lun-tao(河南农业大学牧医工程学院,河南郑州,450002) |
| 摘 要： | 2003年Christensen等将过去归为输卵管炎放线杆菌、禽溶血性巴氏杆菌及鸭巴氏杆菌的细菌归为巴氏杆菌科中的一个新属——卡氏杆菌属。卡氏杆菌主要引起产蛋母鸡发病，可以导致输卵管炎、卵巢炎、腹膜炎、败血症、心包炎、肝炎、肠炎和呼吸道疾病等。 |
| 关 键 词： | 放线杆菌 产蛋母鸡 基因序列分析 分子鉴定 卡氏 rRNA 溶血性巴氏杆菌 16S |
Molecular Identification of Gallibacterium from Hen and Analysis of Its 16S rRNA Gene |
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| HUANGFU He-ping,WANG Chuan-qing,CHEN Lu,YANG Xia,LIU Hong-ying,PENG Zhi-feng,ZHENG Lu-ping,XU Xue,LIU Hui-min,FU Ren-yi,GUO Lun-tao. Molecular Identification of Gallibacterium from Hen and Analysis of Its 16S rRNA Gene[J]. Agricultural Science ＆ Technology, 2009, 10(1): 43-46,55 | |
| Authors: | HUANGFU He-ping WANG Chuan-qing CHEN Lu YANG Xia LIU Hong-ying PENG Zhi-feng ZHENG Lu-ping XU Xue LIU Hui-min FU Ren-yi GUO Lun-tao |
| Affiliation: | 1. College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002; 2. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011) |
| Abstract: | Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity (99.9%) was observed between the isolated strain and one of the strains of Gallibacterium anatis (AF228002), the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI (AF228016, Gallibacterium genomosp.1, AF228017, Gallibacterium genomosp.2, AF228018, Gallibacterium genomosp.1) were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7%-93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis. |
| Keywords: | Hen Gallibacterium Isolation and identification Homology analysis |
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