Use of a monoclonal antibody in the diagnosis of infection by Dermatophilus congolensis |
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| Authors: | S J How D H Lloyd J Lida |
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| Institution: | Department of Veterinary Medicine and Animal Husbandry, Royal Veterinary College, North Mymms, Hertfordshire. |
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| Abstract: | A monoclonal antibody (McAb) to Dermatophilus congolensis was produced from murine hybridoma cultures and purified by affinity chromatography. Species specificity was demonstrated using indirect immunofluorescent staining; the McAb was shown to react with 10 D congolensis isolates but not with 10 Nocardia species isolates, a Rhodococcus and a Streptomyces species isolate. The McAb was used to demonstrate D congolensis in clinical material from confirmed bovine and ovine cases and presumptive equine cases of dermatophilosis by indirect immunofluorescent staining. |
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