回流提取川楝子中川楝素工艺的优化

目的]优化川楝子中川楝素溶剂回流提取法的最佳提取工艺。方法]采用HPLC法检测,以川楝素含量作为指标,考察提取溶剂、料液比、提取温度、提取时间、提取次数对川楝素提取率的影响,筛选出最佳的提取工艺。结果]溶剂回流法的最佳条件：以甲醇为溶剂,料液比为1∶30,提取温度为80℃,提取时间为1.0 h,提取次数为2次。在最佳工艺条件下,川楝素提取率达1.344 9 mg/g。结论]该工艺简单、稳定、可行,为川楝素的提取提供了一种有效的方法。

Optimization of Reflux Extraction Process for Toosendanin from FRUCTUS TOOSENDAN

Objective] To obtain the optimum reflux extraction conditions for toosendanin from FRUCTUS TOOSENDAN.Method] Contetn of toosendanin was determined by HPLC,and effects of extraction solvent,solid to liquid ratio,extraction temperature,extraction duration and extraction times on yield of toosendanin were investigated using content of toosendanin as index.Result] The optimal extraction conditions for extracting toosendanin by reflux extraction were proposed to be: extraction solvent of methanol,solid to liquid ratio of 1∶30,extraction temperature of 80 ℃,extraction duration of 1.0 h,extraction times of two.Under the optimum extraction process,the extraction rate of toosendanin from FRUCTUS TOOSENDAN reached 1.344 9 mg/g.Conclusion] Simple in operation,the stable reflux extraction established in this study is an effective method for extracting toosendanin from FRUCTUS TOOSENDAN.