用血清酸滴定法对3个品种绵羊进行早期妊娠诊断的研究

目的]为了建立一种快速简易的绵羊早期妊娠诊断方法,提高母羊的繁殖效率,降低繁殖成本。方法]选择1 317只母羊,为新疆地方品种多浪羊、策勒羊和卡拉库尔羊。于人工授精配种后16～30 d,采用血清酸滴定法进行了早期妊娠诊断。结果]在试验绵羊中,共检出妊娠母羊1 128只,未妊娠羊189只。与实际妊娠结果相比,妊娠符合率为89.88%（1 013/1 128）,未孕符合率为96.83%（183/189）,总符合率为90.81%（1 196/1 317）。经卡方检验,这些指标在3个品种母羊或2个品种母羊间差异均不显著。结论]血清酸滴定法诊断时间早、方法简便、结果准确,可以用于绵羊的早孕诊断,极具实用性和推广价值。

Early Pregnancy Diagnosis by Serum Acid Titration in Three Sheep Breeds

Objective] Earlier identification of pregnant and non-pregnant animals post breeding can greatly improve reproductive efficiency and pregnancy rate and decrease the interval between services.The experiment was conducted to develop a rapid and simple early pregnancy diagnostic method in sheep.Method] The early pregnancy diagnosis was conducted with serum acid titration in 1 317 female sheep(Duolang sheep,Cele sheep and Karakul sheep) after 16-30 d post artificial insemination.Result] After the serum acid titration,1 128 pregnant ewes and 189 non-pregnant ewes were detected in the three sheep breeds.Compared with the actual pregnancy detection,the coincidence rate of pregnancy was 89.88%(1 013/1 128);the coincidence rate of non-pregnancy was 96.83%(183/189);and the total coincidence rate was 90.81%(1 196/1 317).Chi-square test analysis showed that no significant difference(P<0.05) was found between the three sheep breeds or between any two breeds.Conclusion] This serum acid titration is a simple,convenient and highly accurate method for early pregnancy diagnosis in sheep,which shows highly practical and popularizing value.