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| 剑麻皂素的分离纯化工艺研究 | |
| 引用本文： | 周寅,张梦宇,白杨,贾桂云. 剑麻皂素的分离纯化工艺研究[J]. 安徽农业科学, 2011, 39(8): 4540-4542 |
| 作者姓名： | 周寅 张梦宇 白杨 贾桂云 |
| 作者单位： | 海南师范大学化学与化工学院,海南海口,571158;海南师范大学化学与化工学院,海南海口,571158;海南师范大学化学与化工学院,海南海口,571158;海南师范大学化学与化工学院,海南海口,571158 |
| 基金项目： | 海南师范大学国家大学生创新实验计划资助项目(00203012702) |
| 摘 要： | [目的]研究从剑麻（Agave sisaiana Perrine）中提取与分离纯化剑麻皂素的工艺。[方法]采用常规的硫酸加热回流提取法,提取剑麻皂素粗提物,用大孔径树脂分离纯化剑麻皂素,并确定最佳提纯工艺条件。[结果]用D-101树脂分离效果较好,最佳分离纯化工艺条件是,洗脱液浓度为70%乙醇,用量100 ml,洗脱速度0.6 ml/min。在此条件下,剑麻皂素得率为79.1%,纯度为71.6%。[结论]该试验研究工艺技术上可行,原料价廉适合工业化生产。 |
| 关 键 词： | 剑麻皂素 大孔树脂 提取 分离 高效液相色谱法 |
Study on Technology of Isolation and Purification of Tigogenin |
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| Affiliation: | ZHOU Yin,ZHANG Meng-yu (College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158) |
| Abstract: | [Objective]The research aimed to study the technology of extraction,isolation and purification of tigogenin from Agave sisalana Perr.ex Engelm.[Method] The crude extracts of tigogenin were extracted by using conventional heating reflux extraction method with sulphuric acid,isolated and purified by macroporous resin,and the optimum technology was determined.[Results] It was better to isolate tigogenin by using D-101 resin.The recovery rate and purity were respectively 79.1% and 71.6% under the optimum conditions,which were isolated and purified at the speed of 0.6 ml/min with 100 ml of 70% alcohol as eluent.[Conclusion] The experimental method was feasible for industrial production of tigogenin due to reasonable technique method and lower raw materials, |
| Keywords: | Tigogenin Macroporous resin Extraction Isolation and purification HPLC |
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