| 药食昆虫九香虫AFLP遗传多样性研究 |
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| 引用本文： | 范钦,朱刚利,孙学刚,蔡红兵,廉振民,王启瑞.药食昆虫九香虫AFLP遗传多样性研究[J].安徽农业科学,2011(8):4732-4733,4767. |
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| 作者姓名： | 范钦 朱刚利 孙学刚 蔡红兵 廉振民 王启瑞 |
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| 作者单位： | 南方医科大学中医药学院;广东环境保护工程职业学院环境工程系;延安大学 |
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| 基金项目： | 广东省中医药局科研课题(2009263,2010223) |
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| 摘 要： | 目的]对广州鼎湖山和罗浮山药食昆虫九香虫（Aspongopus chinensis Dallas）种群进行遗传多样性分析。方法]利用扩增片段长度多态性（AFLP）标记,建立九香虫AFLP指纹图谱,分析其种群遗传多样性参数。结果]4个引物组合共揭示了362个位点,其中293个位点具有多态性,多态性位点所占比例为80.94%。种群Nei＇s基因多样性指数平均为0.2467,Shannon多态性信息指数为0.3687,种群间基因分化系数GST为0.1701,种群每代迁移数Nm为3.157。结论]AFLP标记具有很高的多态性检测效率,适合用于分析九香虫种群遗传多样性;九香虫种群内的遗传变异程度较高,种群具有丰富的DNA遗传多样性水平。
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| 关 键 词： | 半翅目 九香虫 扩增片段长度多态性 遗传多样性 |
AFLP Analysis on the Genetic Diversity of Physic-edible A.chinensis Dallas |
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| Institution: | FAN Qin et al (College of traditional Chinese medicine,Southern Medical University,Guangzhou,Guangdong 510515) |
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| Abstract: | Objective] To study the genetic diversity of the physic-edible A.chinensis Dallas in Dinghu Mountain and Luofu Mountain of Guangzhou.Methods]The fingerprint of A.chinensis Dallas was established through amplified fragment length polymorphism(AFLP),so as to analyze the genetic diversity parameters of A.chinensis.Results] 362 clear bands with 293 polymorphic ones were obtained from 4 primer combinations,and the polymorphic bands covered 80.94% of total.Nei′s genetic diversity index was 0.2467,Shannon polymorphic information index was 0.368 7,the gene differentiation index(GST) among the populations was 0.170 1,and the migration number of each generation was 3.157(Nm).Conclusion] The results showed that AFLP analysis was of high efficiency and suitable for analyzing the genetic diversity of A.chinensis Dallas;Great genetic variation and diversity exists in the population of A.chinensis. |
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| Keywords: | Hemiptera A chinensis Dallas AFLP Genetic diversity |
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