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| 魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别 | |
| 引用本文： | 王洪建,郭斌. 魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别[J]. 安徽农业科学, 2011, 0(8): 4734-4735 |
| 作者姓名： | 王洪建 郭斌 |
| 作者单位： | 辽宁医学院;辽宁医学院附属一院 |
| 基金项目： | 辽宁省教育厅基金项目(202173371) |
| 摘 要： | [目的]用聚丙烯酰胺凝胶电泳对魟鱼软骨多糖（RCG）进行定性鉴别。[方法]利用纯化RCG,进行聚丙烯酰胺凝胶电泳。[结果]RCG经纯化,基本实现了多糖的分离;根据聚丙烯酰胺凝胶电泳迁移率,可明显鉴别出魟鱼RCG。[结论]该法重现性较好,简单易行,可用于魟鱼RCG的定性鉴别。 |
| 关 键 词： | 魟鱼软骨多糖 纯化 聚丙烯酰胺凝胶电泳 鉴别 |
Identification of Ray Cartilage Glycosaminoglycans by Polyacrylamide Gel Electrophoresis |
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| Affiliation: | WANG Hong-jian et al(Liaoning Medical University,Jinzhou,Liaoning 121001) |
| Abstract: | [Objective] This paper aims to study the feasibility of qualitative determination of Ray Cartilage Glycosaminoglycans(RCG) by polyacrylamide gel electrophoresis.[Methods] Pure RCG is applied to conduct phosphate buffer of polyacrylamide gel electrophoresis.[Results] After being purified,RCG realizes seclusion of amylase.According to the electrophoretic mobility of polyacrylamide gel electrophoresis,RCG could be obviously identified.[Conclusions] This method has good repeatability and could be used for the qualitative identification of RCG. |
| Keywords: | Ray Cartilage Glycosaminoglycans Purification Polyacrylamide gel electrophoresis Identification |
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