番茄青枯病病原菌拮抗菌株的筛选及其田间防控作用研究

从番茄青枯病发病严重田块的健康植株根际土壤中分离筛选得到2株高效拮抗菌株,命名为W12和W118,经16SrDNA基因鉴定均属芽胞杆菌属;用PCR扩增的方法扩增脂肽类抗生素合成基因,结果表明W12和W118含有合成bacillomycin、iturin和fengycin三种抗生素的基因;将2株拮抗菌用于田间试验,结果表明混合菌株防控效果最好,3次灌菌后防控效果达到62.3%,单独施用菌株W118较单独施用W12防控效果好,3次灌菌后防控效果达到56.7%。

Screening and identifying of antagonistic bacteria against Ralstonia solanacearum and the control effects on tomato bacterial wilt in the field

Adelphocoris suturalis overwinters with diapause eggs laid in the stems of cotton,tissue of weed,fruit trees and alfalfa hosts adjacent to the cotton fields,which is the most critical link of its life history.To identify the period of the embryo development stagnation in diapause eggs,we performed a morphological observation using microscope and paraffin section.Eggs,nymphs and adults were reared on green beans at (26±1)℃,70%± 10% relative humidity,and a photoperiod cycle of 14 h light/10 h dark.The embryo stopped growing within 48 hours after oviposition in diapause eggs.While in the non-diapause eggs,the embryo kept developing with cells differentiation,9 days after oviposition,the nymphs were hatched.The weight of non-diapause eggs continued to increase with the development of embryo.However,the weight of diapause eggs maintained stability after the formation of diapause.