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铁皮石斛茎基部2种主要病害病原菌的分离与鉴定
引用本文:王国荣,沈伟东,孙 超,沈肖玲,邱春英,苏珍珠,楼兵干.铁皮石斛茎基部2种主要病害病原菌的分离与鉴定[J].植物保护,2017,43(1):168-172.
作者姓名:王国荣  沈伟东  孙 超  沈肖玲  邱春英  苏珍珠  楼兵干
作者单位:1. 四川省农业科学院经济作物育种栽培研究所,成都,610300;2. 四川省烟草公司凉山州公司,西昌,615000;3. 四川省农业科学院植物保护研究所,成都,610066;4. 四川省农业科学院园艺研究所,成都,610066;5. 四川省烟草公司,成都,610041
基金项目:四川省财政现代农业创新与示范项目(2015JSCX-008); 四川省烟草公司科技项目(SCYC201402001C)
摘    要:铁皮石斛茎基部病害是铁皮石斛的重要病害,严重影响铁皮石斛的产量和质量。为了明确引起浙江铁皮石斛茎基部主要病害的病原菌,采集具典型症状的发病植株进行病原菌的分离、纯化、病原菌形态学特征观察、rDNAITS序列分析、致病性试验结合田间症状,明确浙江铁皮石斛茎基部主要病害是由齐整小核菌Sclerotiumrolfsii引起的白绢病和由尖孢镰刀菌Fusarium oxysporum引起的枯萎病。两种病害都侵染茎基部和茎,并最终导致整株死亡,区别是白绢病发病初期病部通常呈水渍状、褪绿、略肿大、有时伴有黄色液体渗出,后腐烂枯死;枯萎病初期茎秆褪绿、萎蔫,后缢缩干枯而死。湿度大时,前者病部遍布白色绢状菌丝体;后者病部出现白色及粉红色霉状物。

关 键 词:黄秋葵  棉蚜  棉平突蚜茧蜂  瓢虫  草蛉  食蚜蝇
收稿时间:2016/2/29 0:00:00
修稿时间:2016/4/3 0:00:00

Isolation and identification of two main basal stem pathogens on Dendrobium officinale
Wang Guorong,Shen Weidong,Sun Chao,Shen Xiaoling,Qiu Chunying,Su Zhenzhu,Lou Binggan.Isolation and identification of two main basal stem pathogens on Dendrobium officinale[J].Plant Protection,2017,43(1):168-172.
Authors:Wang Guorong  Shen Weidong  Sun Chao  Shen Xiaoling  Qiu Chunying  Su Zhenzhu  Lou Binggan
Institution:1. Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610300, China; 2. Liangshan Municipal Tobacco Company, Xichang 615000, China; 3. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 4. Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 5.Sichuan Tobacco Company, Chengdu 610041, China
Abstract:The aphid and their natural enemies on okra in Chengdu were investigated during 2014 and 2015, and the occurrence dynamics of dominant enemies was studied systematically. The results indicated that the aphid on okra is mainly Aphis gossypii Glover. There were 21 species of natural enemies, which belonged to 8 families of 5 orders, and the dominant ones were Lysiphlebia japonica (Ashmead), Harmonia axyridis (Pallas), Propylea japonica (Thunberg) and Chrysopa pallens (Rambur). The numbers of aphid peaked 40 days after transplanted, and then decreased gradually.The occurrence dynamics of L. japonica had a strong following relationship with that of aphid.There was obvious difference among the dynamic of ladybugs, lacewings and hoverflies, and their following relationship of occurrence dynamic was not obvious with that of aphid.
Keywords:Abelmoschus esculentus  Aphis gossypii  Lysiphlebia japonica  ladybug  lacewing  hoverfly
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