秋石斛离体快速繁殖技术研究

以秋石斛‘三亚阳光’幼芽为外植体,采用丛生芽诱导途径,并利用正交设计法,探讨基本培养基(花宝1号、改良1#、改良2#、改良3#)、植物生长调节剂(6-BA、NAA、IBA)、白糖等对其丛生芽诱导、增殖、生根等关键环节的影响,以期建立秋石斛‘三亚阳光’丛生芽组培快繁技术。结果表明:各试验因素对秋石斛丛生芽增殖影响的主次关系为基本培养基6-BANAA白糖;筛选出丛生芽适宜的增殖培养基配方为改良2#+6-BA 1.0mg·L~(-1)+NAA 0.2mg·L~(-1)+白糖30.0g·L~(-1)+琼脂粉3.0g·L~(-1)+卡拉胶3.0g·L~(-1),50d平均增殖系数达6.25;筛选出适宜生根的培养基配方为改良3#+NAA 0.3mg·L~(-1)+IBA 0.5mg·L~(-1)+活性炭0.5g·L~(-1)+香蕉泥100.0g·L~(-1)+白糖20g·L~(-1)+琼脂粉3.6g·L~(-1)+卡拉胶3.6g·L~(-1),生根率为100.0%;试管苗移栽60d成活率达96.5%。

Rapid Propagation of Dendrobium spp.Using Tissue Culture

Newly geminated buds of Dendrobium spp.,Sanya Sunny,were used asexplants to induce bud clumps.Effects of culture media (i.e.,Hyponex # 1,Improvement # 1,Improvement # 2,and Improvement # 3),phytohormones (i.e.,6-BA,NAA,and IBA),and sugar on the bud clump induction,propagation,and rooting of the plantlets were analyzed and optimized using anorthogonal experiment.The results showed that the major factors affecting the bud multiplication were medium,6-BA,NAA,and sugar.Improvement #2 medium that contained 1.0 mg · L-1 of 6-BA,0.2 mg · L-1 of NAA,30.0 g · L-1 of sugar,3.0 g · L-1 of agar powder and 3.6 g · L-1 of carrageenanprovided the best average propagation coeficient of 6.25 in 50 d among all tested media.For rooting,Improvement # 3 with added NAA at 0.3 mg · L-1,IBA at 0.3 mg * L-1,AC at 0.5 g · L-1,banana puree at 100.0 g · L-1,sugar at 20.0 g · L-1,agar powder at 3.6 g · L-1 and carrageenan at 3.6 g · L-1 performed best with a 100.0％ rooting rate.The survival rate of the plantlets in 60 d after transplanting from test tubes was 96.5％.