| 茶叶多糖的组合及免疫活性研究 |
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| 引用本文： | 汪东风 李俊. 茶叶多糖的组合及免疫活性研究[J]. 茶叶科学, 2000, 20(1): 45-50 |
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| 作者姓名： | 汪东风 李俊 |
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| 作者单位： | [1]安徽农业大学农业部茶叶生物技术重点开放实验室 [2]安徽医科大学临床药理研究所 |
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| 基金项目： | 安徽省自然科学基金! (971 2 0 0 4)项目内容之一 |
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| 摘 要： | 用粗老茶制备要多糖（ＴＰＳ）的粗品（ＴＰＳ１）经脱蛋白处理过凝胶柱纯后得ＴＰＳ的纯品（ＴＰＳ２），然后用ＵＶ、ＩＲ、ＨＰＬＣ、ＧＣ及常规成分分析等方法比较了ＴＰＳ１和ＴＰＳ２之间的组分差异；用ＡＡ（佐剂性关节炎）大鼠和正常小鼠为对象。比较ＴＰＳ１和ＴＰＳ２对动物免疫活性的影响。
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| 关 键 词： | 茶叶多糖 组成 免疫 |
Study on the Component and Immune Activity of Polysaccharides from Tea |
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| WANG Dong feng ,,LI Jun ,WANG Chang hong ,ZHAO Gui wen JIN Yong ,CHENG Ding ding ,YE Sheng [WTBX]. Study on the Component and Immune Activity of Polysaccharides from Tea[J]. Journal of Tea Science, 2000, 20(1): 45-50 |
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| Authors: | WANG Dong feng LI Jun WANG Chang hong ZHAO Gui wen JIN Yong CHENG Ding ding YE Sheng [WTBX] |
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| Affiliation: | WANG Dong feng 1,3,LI Jun 2,WANG Chang hong 1,ZHAO Gui wen 3 JIN Yong 2,CHENG Ding ding 1,YE Sheng 1 [WT6BX] |
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| Abstract: | Crude polysaccharides(TPS 1)was made from coarse tea by previous methods,and the pure polysaccharides(TPS 2)was purified from TPS 1 by deproteining and through the SephadexG 75 and SephadexG 150 columns The components and immune activity of TPS 1 and TPS 2 were determined and compared with UV,IR,GC,HPLC and conventional methods Results showed that the protein in TPS was conjugated with the polysaccharides TPS is a glycoprotein The TSP showed the recovering effects on the low transforming rate of spleen lymphocytes and the low secretion of interlukin 2(LI 2) in AA rats.Besides,it showed the inhibitory effects on the too high secretion of interlukin 1(IL 1).The immune function of normal mice could be enhanced by TPS The effects of TPS 2 was better than those of TPS 1 |
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| Keywords: | Tea polysaccharide Preparing methods Component Immunization |
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