| 陆地棉花粉母细胞时期microRNAs分析 |
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| 引用本文： | 魏娟,吴建勇,邢朝柱,郭立平,戚廷香,王海林.陆地棉花粉母细胞时期microRNAs分析[J].棉花学报,2011,23(5):433-439. |
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| 作者姓名： | 魏娟 吴建勇 邢朝柱 郭立平 戚廷香 王海林 |
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| 作者单位： | 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳,455000 |
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| 基金项目： | 转基因生物新品种培育重大专项(2008ZX08005-001) |
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| 摘 要： | 以陆地棉花粉母细胞时期的小蕾为材料,利用Solexa测序技术和miRNA相关的生物信息学分析技术,共得到151条单一miRNA序列,其中已知植物中保守miRNAs有88个,新发现的棉花miRNAs有2个,候选棉花miRNAs有61个.通过miRNA靶基因预测和靶基因功能分析发现6个与陆地棉花发育相关的miRNAs和5个...
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| 关 键 词： | Solexa测序 棉花miRNAs 花粉母细胞 雄配子发育 |
Analysis of microRNAs at Cotton Microsporocyte Stage |
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| WEI Juan,WU Jian-yong,XING Chao-zhu,GUO Li-ping,QI Ting-xiang,WANG Hai-lin.Analysis of microRNAs at Cotton Microsporocyte Stage[J].Cotton Science,2011,23(5):433-439. |
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| Authors: | WEI Juan WU Jian-yong XING Chao-zhu GUO Li-ping QI Ting-xiang WANG Hai-lin |
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| Institution: | WEI Juan,WU Jian-yong,XING Chao-zhu,GUO Li-ping,QI Ting-xiang,WANG Hai-lin(Cotton Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Cotton Genetics Improvement,MOA,Anyang,Henan 455000,China) |
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| Abstract: | In this research,the buds of Gossypium hirsutum at microsporocyte stage were used as materials.The Solexa sequencing and miRNA related bioinformatics were performed to analyze miRNAs in upland cotton.Finally,151 unique miRNAs sequence were obtained.They include 88 known miRNAs,2 new cotton miRNAs and 61 candidate cotton miRNAs.In addition,6 flower-developing related miRNAs and 5 male gametogenesis-related miRNAs were found through miRNA target prediction and function analysis. |
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| Keywords: | Solexa sequencing cotton miRNAs microsporocyte male-gametogenesis |
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