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亚麻种质资源遗传多样性与亲缘关系的AFLP分析
引用本文:李明.亚麻种质资源遗传多样性与亲缘关系的AFLP分析[J].作物学报,2011,37(4):635-640.
作者姓名:李明
作者单位:东北农业大学农学院,黑龙江哈尔滨150030
基金项目:黑龙江省博士后留省工作启动基金
摘    要:选取18个中国品种和67份来自世界各地的亚麻材料(包括油用亚麻,农家种和野生种),采用7对引物通过扩增片段长度多态性(AFLP)分子标记分析, 获得168个位点具有多态性,平均每对引物可扩增出24条带。利用NTSYS 2.1采用非加权配对算术平均法(UPGMA)方法聚类分析,计算遗传距离和构建遗传系统进化树。结果显示85份材料在遗传相似系数0.53处分为4类,可以将栽培种和3个野生种区分开来;在遗传相似系数0.67处可以将纤维亚麻划为1类,而绝大多数油用亚麻分成4类,油用亚麻的遗传多样性远高于纤维亚麻;国产纤维亚麻中黑字号品种相似度极高,遗传背景狭窄,而双字号品种多样性稍好。还证明Linum bienne与亚麻栽培种(Linum usitatissimum L.)关系密切,并支持纤维亚麻来自油用亚麻的假说。

关 键 词:亚麻种质资源  遗传多样性  亲缘关系  AFLP  
收稿时间:2010-07-21

Genetic Diversity and Relationship of Flax Germplasm as Revealed by AFLP Analysis
LI Ming.Genetic Diversity and Relationship of Flax Germplasm as Revealed by AFLP Analysis[J].Acta Agronomica Sinica,2011,37(4):635-640.
Authors:LI Ming
Institution:Flax Laboratory of Northeast Agricultural University, Harbin 150030, China
Abstract:Flax (linum usitatissimum) is one of the major fibre and oil yielding crops. AFLP is very useful molecular marker for a number of plant species. To reveal the genetic diversity and find ways to broaden the genetic background of Chinese fibre flax varieties, we analyzed 18 Chinese fibre flax cultivars and other 67 accessions (including linseed, landrace and wild species) from different countries in the world by using AFLP marker. In total, 168 polymorphic alleles were detected with seven pairs of AFLP primer...
Keywords:AFLP
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