| 用T-DNA插入突变的方法获得稻瘟病菌致病突变体 |
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| 引用本文： | 李亚,孔慧敏,梁慎,方敏彦,阎霞,王政逸.用T-DNA插入突变的方法获得稻瘟病菌致病突变体[J].浙江农业学报,2008,20(6). |
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| 作者姓名： | 李亚 孔慧敏 梁慎 方敏彦 阎霞 王政逸 |
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| 作者单位： | 1. 浙江大学,农业与生物技术学院,生物技术研究所,浙江,杭州,310029
2. 甘肃农业大学,草业学院,甘肃,兰州,730070 |
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| 基金项目： | 国家自然科学基金资助项目
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| 摘 要： | 利用农杆菌介导转化的方法共得到1 114个稻瘟病菌转化子,经感病大麦和水稻离体叶片接种鉴定获得3个致病缺陷或下降突变体:A1-134、A1-452、和A2-1-8,其中A1-134和A1-452的致病性下降,而A2-1-8突变体的致病性丧失.对这些突变体的分生孢子形态、产孢、附着胞形成等表型分析的结果发现,A1-134突变体的产孢量显著下降,仅为野生菌株Guy11的7.7%,分生孢子为圆球形,无隔膜或只有一个隔膜;A1-452产孢量明显增多,为Guy11的22倍;A2-1-8的分生孢子为长针形,产孢量略有下降;A1-134和A1-452在疏水表面的附着胞形成率与Guy11相近,而A2-1-8则不能形成附着胞.研究结果为进一步鉴定和分离这些突变体的T-DNA标记基因奠定了基础.
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| 关 键 词： | 稻瘟病菌 根癌农杆菌介导转化 T-DNA插入突变 致病突变体 |
Identification of pathogenicity mutants of Magnaporthe grisea by T-DNA insertional mutagenesis |
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| LI Ya,KONG Hui-min,LIANG Shen,FANG Min-yan,YAN Xia,WANG Zheng-yi.Identification of pathogenicity mutants of Magnaporthe grisea by T-DNA insertional mutagenesis[J].Acta Agriculturae Zhejiangensis,2008,20(6). |
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| Authors: | LI Ya KONG Hui-min LIANG Shen FANG Min-yan YAN Xia WANG Zheng-yi |
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| Institution: | LI Ya1,KONG Hui-min1,LIANG Shen1,FANG Min-yan2,YAN Xia1,WANG Zheng-yi1(1Biotechnology Institute,College of Agriculture , Biotechnology,Zhejiang University,Hangzhou 310029,PR China,2College of Grassl, Science,Gansu Agriculture University,Lanzhou 730070,China) |
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| Abstract: | 1114 transformants of Magnaporthe grisea were obtained using Agrobacterium tumefaciens-mediated transformation.Three pathogenicity mutants,A1-134,A1-452 and A2-1-8,were identified by susceptible barley and rice cut-leaf assay.Pathogenicity of A1-134 and A1-452 was reduced,and A2-1-8 was nonpathogenic to both barley and rice.Phenotypic analysis of these mutants showed that the A1-134 produced spherical conidia with one septum or none at all and conidiation was significantly reduced,only 7.7% conidia compared... |
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| Keywords: | Magnaporthe grisea Agrobacterium tumefaciens-mediated transformation insertional mutagenesis mutant |
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