| 侧金盏花组织培养及无性系建立的研究 |
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| 引用本文： | 李欢,肖筝,王本全,李铁松,姜长阳.侧金盏花组织培养及无性系建立的研究[J].特产研究,2010,32(1):22-24,28. |
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| 作者姓名： | 李欢 肖筝 王本全 李铁松 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁师范大学教学改革项目 |
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| 摘 要： | 以生长旺盛的侧金盏花嫩茎腋芽为材料,进行了芽的生长、分化及试管苗的生根、移栽、扦插和移植研究,建立起侧金盏花的无性系。结果证明:1/2MS+IAA0.5mg/L+NAA0.1mg/L是芽生长培养的理想培养基;MS+BA0.8mg/L+NAA0.3mg/L是生长芽分化培养和不定芽继代培养的理想培养基;1/2MS+NAA0.1 mg/L+IAA0.4 mg/L是不定芽生根培养和生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。试管苗移植后长势整齐、旺盛,各种生物学性状均与野生植株一致。
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| 关 键 词： | 侧金盏花 组织培养 无性系 |
Tissue Culture and Establishment of Asexual Line for Adonis amurensis |
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| LI Huan,XIAO Zheng,WANG Ben-quan,LI Tie-song,JIANG Chang-yang.Tissue Culture and Establishment of Asexual Line for Adonis amurensis[J].Special Wild Economic Animal and Plant Research,2010,32(1):22-24,28. |
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| Authors: | LI Huan XIAO Zheng WANG Ben-quan LI Tie-song JIANG Chang-yang |
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| Institution: | LI Huan,XIAO Zheng,WANG Ben-quan,LI Tie-song,JIANG Chang-yang(College of Life Science,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | A study on growth,differentiation,rooting and transplanting of axillary buds were studied and establishment of asexual sys-tem of Adonis amurensis was also conducted.The results indicated that the ideal media for development of growing point was 1/2MS+IAA0.5mg.L-1+NAA 0.1mg.L-1.The best medium for differentiation was MS+6-BA0.8mg.L-1+NAA0.3mg.L-1.The medium of 1/2MS+NAA0.1mg.L-1+IAA0.4mg.L-1 was suitable for rooting.The cinder was the ideal matrix for plantlet culture with vigorous and variant characters. |
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| Keywords: | Adonis amurensis tissue culture clone |
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