坡柳皂苷对大菜粉蝶生长发育的抑制活性

采用浸渍叶碟饲喂法测定了坡柳皂苷对大菜粉蝶的生物活性。结果表明:用500、1 625、2 750、3 875和5 000μg/mL的坡柳皂苷处理24 h后,对大菜粉蝶4龄幼虫生长发育的抑制率分别为14.47%、31.54%、49.70%、62.48%和79.94%,处理48 h后的抑制率分别为2.72%、39.26%、54.82%、70.53%和86.39%;坡柳皂苷对大菜粉蝶4龄幼虫具有显著的拒食活性,供试5种浓度下处理24 h后,拒食率分别为30.21%、40.62%、54.33%、72.47%和84.84%,48 h后的拒食率为4.4%~86.67%;5 000μg/mL处理14 d后,幼虫化蛹率为32%,显著低于对照组(72%);供试5种浓度坡柳皂苷处理23 d后,蛹的羽化率分别为56%、44%、44%、36%和32%,均低于对照组(64%)。研究表明,坡柳皂苷对大菜粉蝶生长发育、取食及化蛹均有明显的抑制作用。

Inhibitory activity of Dodonaea viscosa saponin on the growth anddevelopment of Pieris brassicae

The biological activity of Dodonaea viscosa saponin to Pieris brassicae was determined by leaf dipping method. The results showed that the growth inhibition rates of 500, 1 625, 2 750, 3 875 and 5 000 μg/mL D.viscosa saponin treatments on the 4th instar larvae of P.brassicae were 14.47%, 31.54%, 49.70%, 62.48% and 79.94% respectively, at 24 h, and were 2.72%, 39.26%, 54.82%, 70.53% and 86.39% respectively, at 48 h. D.viscosa saponin had significantly antifeedant activity on the 4th instar larvae of P.brassicae, and the antifeedant rates of the 5 treatments of D.viscosa saponin mentioned above were 30.21%, 40.62%, 54.33%, 72.47% and 84.84% respectively, at 24 h, and were 4.4%-86.67% at 48 h. The P.brassicae larvae pupate rate of 5 000 μg/mL treatment was 32% after 14 d, which was significantly lower than that of the control (72%). And the P.brassicae emergence rate of the 5 treatments of D.viscosa saponin mentioned above were 56%, 44%, 44%, 36% and 32% respectively, after 23 d, which was lower than that of the control (64%). The results showed that D.viscosa saponin had significant inhibitory activity on growth, development, feeding and pupation of P.brassicae.