Fructooligosaccharide supplementation in diets for tropical gar (Atractosteus tropicus) juvenile: Effects on morphophysiology and intestinal barrier function |
| |
Authors: | Cesar Antonio Sepúlveda‐Quiroz Emyr Saul Pea‐Marín Alfredo Prez‐Morales Rafael Martínez‐García Carina Shianya Alvarez‐Villagomez Claudia Ivette Maytorena‐Verdugo Susana Camarillo‐Coop Paula Gabriela Vissio Daniela Prez Sirkin Dariel Tovar‐Ramírez Mario Galaviz Carlos Alfonso lvarez‐Gonzlez |
| |
Institution: | Cesar Antonio Sepúlveda‐Quiroz,Emyr Saul Peña‐Marín,Alfredo Pérez‐Morales,Rafael Martínez‐García,Carina Shianya Alvarez‐Villagomez,Claudia Ivette Maytorena‐Verdugo,Susana Camarillo‐Coop,Paula Gabriela Vissio,Daniela Pérez Sirkin,Dariel Tovar‐Ramírez,Mario Galaviz,Carlos Alfonso Álvarez‐González |
| |
Abstract: | Supplementary fructooligosaccharides (FOS) were evaluated on growth, survival, gut morphology, digestive enzyme activity and expression of intestinal barrier genes in tropical gar (Atractosteus tropicus). Four treatments were designed to include FOS (5, 10, 15 and 20 g/Kg) and were compared with a control (0 g/Kg) and the current commercial trout diet. Ten fish were stocked (0.25 ± 0.01 g and 4.2 ± 0.4 cm total length) per experimental unit by triplicate in a 45‐day experiment. Fish fed with 5 and 10 g/Kg of FOS had the highest average weight and total average length. Fish fed 5 g/Kg FOS showed the best growth indexes (absolute weight gain: 2.16; specific growth rate: 4.96; and feed conversion rate: 1.23) and survival (96.66%) and also had the highest acid protease, chymotrypsin and leucine aminopeptidase activities. Fish fed 5 g/Kg FOS had a high absorption area and accumulation of lipids in the liver. Finally, relative overexpression of occluding (occ), nucleotide‐binding oligomerization domain‐2 (nod2) and mucin 2 (muc2) was detected in fish fed 10 and 15 g/Kg FOS. The supplementation between 5 and 10 g/Kg FOS in diets for A. tropicus juveniles could be beneficial for growth, digestive enzyme activities, gut morphology and intestinal barrier function. |
| |
Keywords: |
Atractosteus tropicus
digestive enzymes histology intestinal barrier function nutrition prebiotics |
|
|