Investigation of mealybug (hemiptera: Coccoidea: Pseudococcidae) species from Turkey by RAPD |
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| Authors: | Ç Uluba? Serçe M B Kaydan A N Kilinçer F Ertunç |
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| Institution: | (1) Plant Protection Department, Agriculture Faculty, Mustafa Kemal University, Antakya, Turkey;(2) Plant Protection Department, Agriculture Faculty, Yüzüncü Yıl University, 65080 Van, Turkey;(3) Plant Protection Department, Agriculture Faculty, Ankara University, 06610 Dışkapı, Ankara, Turkey |
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| Abstract: | Molecular markers are commonly used for determining the relationship between insect species. In this study, we investigated
the relationship of six mealybug species,Planococcus citri (Risso),Planococcus ficus (Signoret),Planococcus vovae (Nasonov),Pseudococcus longispinus (Targioni Tozzetti),Pseudococcus viburni (Signoret) andPhenacoccus aceris (Signoret), sampled from Turkey, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Of the 50
RAPD primers tested, 18 identified a total of 256 fragments of which all were polymorphic. Sufficient discrimination betweenP. citri andP. ficus was provided by several RAPD primers. The genetic distance values calculated for each pair-wise comparison ranged from 0.156
to 0.504. Cluster analyses of RAPD data clearly separated the species into two groups.
http://www.phytoparasitica.org posting May 4, 2007. |
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| Keywords: | Mealyburg RAPD Planococcus citri Planococcus ficus Planococcus vovae Pseudococcus longispinus Pseudococcus viburni Phenacoccus aceris |
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