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Structure-activity relationships of pheromonostasis induced by ACCase-inhibitor herbicides in the moth Helicoverpa armigera |
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| Authors: | O. Hanin B. Rubin A. Rafaeli |
| Affiliation: | a Agriculture Research Organization, Volcani Center, Institute for Technology and Storage of Agricultural Products, P.O. Box 6, Bet-Dagan 50250, Israel b Institute of Plant Sciences and Genetics in Agriculture, Weed Science Group, The Hebrew University—Rehovot Campus, P.O. Box 12, Rehovot 76100, Israel c Department of Entomology, Laboratory of Insect Physiology, The Hebrew University—Rehovot Campus, P.O. Box 12, Rehovot 76100, Israel |
| Abstract: | Many moth sex pheromone blends are derived from fatty acids and their production is regulated by a Pheromone Biosynthesis Activating Neuropeptide (PBAN). In prior work we showed that the herbicide Diclofop-acid, an acetyl-coenzyme A carboxylase (ACCase) inhibitor, inhibits PBAN-induced sex pheromone production in vitro. In this work we extend our study showing that several other herbicides, belonging to the 2-aryloxyphenoxypropionate (‘FOP’) and the cyclohexandione-oxime (‘DIM’) families significantly inhibit pheromone production by adult females whilst survival is unaffected by treatment. Enzyme activity in vitro and kinetic analysis revealed a Km of 0.35 μM with Ki values of 0.1 and 0.28 μM due to Tralkoxydim and Diclofop inhibition, respectively. Inhibitory activity on PBAN-induced pheromone production by all herbicides tested revealed a potency order: Tralkoxydim > Clodinafop > Cycloxidim > Haloxyfop > Diclofop > Fenoxaprop > Fluazifop > Quizalofop, Quizalofop being inactive. Differences in inhibition efficiencies may be attributed to different binding sites on the enzyme or to the polarity and solubility of these compounds. |
| Keywords: | Moth sex pheromones PBAN Biosynthesis Herbicides Acetyl-coenzyme A carboxylase |
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