| 几株家蚕蛹油降解细菌的分离鉴定与降解能力测定 |
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| 引用本文： | 崔萍,张楠,张莎莎,国辉,吴金娣,刘训理.几株家蚕蛹油降解细菌的分离鉴定与降解能力测定[J].蚕业科学,2010,36(1):102-108. |
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| 作者姓名： | 崔萍 张楠 张莎莎 国辉 吴金娣 刘训理 |
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| 作者单位： | 山东农业大学,山东泰安,271018;河池学院,广西宜州,546300;山东农业大学,山东泰安,271018 |
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| 摘 要： | 利用稀释培养法从缫丝废水中分离到5株可降解家蚕蛹油的细菌,采用紫外分光光度法测定了其对家蚕蛹油的降解能力,H73、H23、H1、H5和H43菌株在72 h内对家蚕蛹油的降解率分别达到76.36%、62.04%、60.58%、53.85%和51.20%。通过形态特征、生理生化特性测定和16S rDNA序列系统发生分析对各菌株进行鉴定,其中H23、H1和H43为芽孢杆菌属(Bacillus sp.),H5为微杆菌属(Microbacterium sp.),H73为肠杆菌属(Enterobacter sp.)。
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| 关 键 词： | 家蚕蛹油 降解 细菌 形态特征 生理生化特性 16SrDNA序列 降解能力 |
Isolation and Identification of Several Bacterial Strains that Degrade Silkworm Pupal Oil and Determination of Their Degradation Capability |
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| CUI Ping,ZHANG Nan,ZHANG Sha-Sha,GUO Hui,WU Jin-Di,LIU Xun-Li.Isolation and Identification of Several Bacterial Strains that Degrade Silkworm Pupal Oil and Determination of Their Degradation Capability[J].Acta Sericologica Sinica,2010,36(1):102-108. |
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| Authors: | CUI Ping ZHANG Nan ZHANG Sha-Sha GUO Hui WU Jin-Di LIU Xun-Li |
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| Institution: | 1Shandong Agricultural University;Taian Shandong 271018;China;2Hechi University;Yizhou Guangxi 546300;China |
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| Abstract: | Five bacterial strains capable of degrading silkworm pupal oil were isolated from the waste water of a silk reeling factory by dilution plate method.Determination of their degrading capability with UV spectrophotometry indicated that strains H73,H23,H1,H5 and H43 had a degradation rate of 76.36%,62.04%,60.58%,53.85% and 51.20% in 72 hours,respectively.The strains were identified based on morphological characters,physiological and biochemical properties,and phylogenetic analysis on their 16S rDNA sequences.I... |
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| Keywords: | Silkworm pupal oil Degradation Bacterium Morphological character Physiological and biochemical property 16S rDNA sequence Degradation capability |
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