An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes |
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Authors: | Mei Jing Piao Yu Jae Hyun Suk Ju Cho Hee Kyoung Kang Eun Sook Yoo Young Sang Koh Nam Ho Lee Mi Hee Ko Jin Won Hyun |
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Institution: | 1.School of Medicine, Jeju National University, Jeju 690-756, Korea; E-Mails: (M.J.P.); (Y.J.H.); (S.J.C.); (H.K.K.); (E.S.Y.); (Y.S.K.);2.Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea; E-Mail: ;3.Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Korea; E-Mail: |
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Abstract: | The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280–320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components. |
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Keywords: | Bonnemaisonia hamifera human keratinocytes photoprotection reactive oxygen species ultraviolet B |
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