拟南芥抗葱链格孢相关基因的差异表达分析

选取病拟南芥转基因NahG(抗病)、生态型WS-2(抗病)和Shakh-dara(感病)为材料,利用mRNA差异显示技术(DDRT-PCR)对抗、感病材料接种葱链格孢后抗性相关基因的差异表达情况进行了分析,共获得135个与拟南芥抗葱链格孢侵染相关的差异表达片段。反式Northern杂交结果表明,38个差异表达片段为阳性片段,阳性率为28.1%。同源性分析结果表明,拟南芥抗感生态型感染葱链格孢后,诱导了呼吸过程、参与细胞壁构建和蛋白质水解等相关基因的表达。其中,接种3 h诱导表达的WS-2-47序列编码含有SH3保守域的Sla1蛋白,表明拟南芥受葱链格孢侵染后可能是通过寄主细胞壁的改变来提高寄主的抗病性。

Differential expression analysis of pathogensis-related genes in different Arabidopsis ecotypes to Alternaria porri

Ecotype WS-2,transgenic NahG(displayed resistance response) and Shakh-dara(displayed susceptible) were taken as material,the expression of differential genes was studied in resistant and susceptible Arabidopsis ecotypes after being inoculated by Alternaria porri through the method of DDRT-PCR.One hundred and thirty-five differential expressed cDNA fragments were obtained and the reverse Northern blotting result indicated that thirty-eight differential expressed cDNA fragments were positive.The positive ratio was 28.1%.Homologous results displayed that not only respiration-related genes were induced but cytoskeleton-like-related genes controlling protein and proteolysis were also induced.Fragment WS-2-47 was induced 3 h after inoculation and encodes Sla1 protein which contain SH3 conserved domain.It putatived that Arabidopsis cell wall was changed and enhanced the host resistance during the infection of Alternaria porri.