| 花生抗黄曲霉相关ARAhPR10基因克隆及其原核表达 |
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| 引用本文： | 谢纯政,梁炫强,李玲,刘海燕. 花生抗黄曲霉相关ARAhPR10基因克隆及其原核表达[J]. 广西农业生物科学, 2009, 0(2): 237-244 |
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| 作者姓名： | 谢纯政 梁炫强 李玲 刘海燕 |
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| 作者单位： | 广东省农业科学院作物研究所;华南师范大学生命科学学院; |
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| 基金项目： | 国家自然科学基金项目(30571179);;国家863计划(2006AA10Z156;2006AA10A115);;广东省自然科学基金(07117967)共同资助 |
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| 摘 要： | 花生黄曲霉污染已成为制约我国花生及花生制品出口贸易的关键因素。基于花生抗黄曲霉相关EST序列，RT-PCR法克隆花生ARAhPR10基因（gb｜EU661964.1），开放读码框471bp，编码157个氨基酸，分子量16.9kD，等电点5.03。与已报导AhPR10（gb｜AY726607）相似性为49.3％。推导氨基酸序列含有PR10家族的高度保守“P-loop”基序（G＊GG＊G）和Betv1保守疏水结构域“GVALP PTAEK ITFET KLVEG PNGGS IGKLT LKY”，推测ARAhPR10是该花生PR10家族的新成员。其基因组扩增序列长度561bp，两个外显子间存在一个长度为87bp的内含子。原核表达ARAhPR10的融合蛋白约25kD，Ni^＋-NTA树脂亲和纯化后获得电泳单一条带。纯化的ARAhPR10融合蛋白具有体外核酸酶活性，预测其具有抗黄曲霉活性。该基因的克隆及其原核表达融合蛋白的获取为ARAhPR10功能研究奠定了基础。
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| 关 键 词： | 花生 黄曲霉 ARAhPRIO基因 原核表达 |
Cloning and Prokaryotic Expression of ARAhPR10 Gene with Resistance to Aspergillus flavus in Peanut |
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| Xie Chunzheng , Liang Xuanqiang Li Ling Liu Haiyan Crops Research Institute,Guangdong Academy of Agriculture Sciences,Guangzhou,, College of Life Science,South China Normal University. Cloning and Prokaryotic Expression of ARAhPR10 Gene with Resistance to Aspergillus flavus in Peanut[J]. Journal of Guangxi Agricultural and Biological Science, 2009, 0(2): 237-244 |
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| Authors: | Xie Chunzheng Liang Xuanqiang Li Ling Liu Haiyan Crops Research Institute Guangdong Academy of Agriculture Sciences Guangzhou College of Life Science South China Normal University |
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| Affiliation: | Xie Chunzheng 1,2 Liang Xuanqiang 1 Li Ling 2 Liu Haiyan 1 1 Crops Research Institute,Guangdong Academy of Agriculture Sciences,Guangzhou,510640,2 College of Life Science,South China Normal University,510630 |
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| Abstract: | A crucial factor restricting the export of the peanut produce in our country was some peanut with the aflatoxin contamination. Based on the EST related Aspergillus flavus resistant,we obtained a cDNA sequence of ARAhPR10(gb|EU661964.1) from the peanut by RT-PCR. The cDNA of ARAhPR10 contained a single ORF spanning 471 bp. The ORF encoded a predicted polypeptide of 157 amino acid residues,with a predicted molecular mass of 16.9 kD and pI of 5.03. Sequence alignment,using DNAStar MegAlign,of the predicted ami... |
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| Keywords: | Peanut Aspergillus flavus ARAhPR10 gene Prokaryotic expression |
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