| 白兰花组织培养技术的初步研究 |
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| 引用本文： | 陈亚鸿,陈雄庭.白兰花组织培养技术的初步研究[J].广东农业科学,2009(6):46-49. |
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| 作者姓名： | 陈亚鸿 陈雄庭 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海南,海口,571101;海南大学农学院,海南,海口,570228
2. 中国热带农业科学院热带生物技术研究所,海南,海口,571101 |
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| 摘 要： | 以白兰花半木质化的侧芽为外植体,用两种灭菌剂配合使用比单一使用效果要好,污染率从100%下降到20%以下;最佳芽诱导培养基为1/2MS+6-BA1.0mg/L+2,4-D0.1mg/L+KT0.5 mg/L+椰子水5%+蔗糖30g/L+琼脂5.8 g/L;最佳增殖培养基为MS+6-BA1.0mg/L+NAA0.1mg/L+KT0.1mg/L+GA30.1mg/L+蔗糖6%+琼脂粉5.8g/L.
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| 关 键 词： | 白兰花 灭菌剂 诱导 增殖 |
Preliminary research on tissue culture of Michelia alba DC. |
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| CHEN Ya-hong,CHEN Xiong-ting.Preliminary research on tissue culture of Michelia alba DC.[J].Guangdong Agricultural Sciences,2009(6):46-49. |
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| Authors: | CHEN Ya-hong CHEN Xiong-ting |
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| Institution: | CHEN Ya-hong1,2,CHEN Xiong-ting1(1.Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou 571101,China,2.College of Agronomy,Hainan University,Haikou 570228,China) |
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| Abstract: | The disinfection effect was much better by using two kinds of microbicide than one.The contamination rate was lowered from 100% to 20% while disinfecting the semi-lignified lateral buds explants of Michelia alba DC.The best buds induction medium was 1/2MS+6-BA1.0 mg/L+2,4-D0.1 mg/L+KT0.5 mg/L+coconut water 5%+cane sugar 30 g/L+agar 5.8 g/L.The best buds multiplication medium was MS+6-BA1.0 mg/L+NAA0.1 mg/L+KT0.1 mg/L+GA30.1 mg/L+ cane sugar 6%+agar 5.8 g/L. |
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| Keywords: | Michelia alba DC microbicide induction multiplication |
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