刚竹属3种重要散生竹光系统Ⅰ基因（Lhca1）的克隆、序列分析和蛋白结构预测

光系统Ⅰ(PSⅠ)在植物光合系统中具有重要功能，Lhca1是编码PSⅠ复合物中最主要的捕光色素蛋白LHCⅠ的基因。该研究采用RT-PCR法，从毛竹中克隆了捕光叶绿素a/b结合蛋白基因Lhca-Pe01(GenBank EU035496)的全长，从红壳竹和角竹中克隆了长度分别为616、613 bp的捕光叶绿素a/b结合蛋白基因片段LhcaH01(GenBank EU513200、 LhcaJ01(GenBank EU513201)。运用生物信息学方法对其核苷酸序列、编码的氨基酸序列以及蛋白结构进行预测。结果表明，LhcaPe01基因序列从第39 bp开始到第779 bp含有1个开放阅读框和一个中止密码子，该基因全长1 051 bp，在5′端有38 bp的非编码区，在3′端含有242 bp的非编码区和Poly(A) 30 bp。对这3个基因的保守片段的序列分析表明，刚竹属3种竹种毛竹、红壳竹和角竹保守区内核酸序列同源性非常高，在禾本科内不同属之间毛竹与大麦序列同源性最高，玉米次之。编码的氨基酸序列同源性与核酸序列同源性一致，最高是毛竹与大麦，水稻次之。经推测LhcaPe01编码的蛋白质等电点和分子量分别为5.400 0和22 107.34 D。蛋白质结构预测表明，毛竹、大麦、水稻、玉米的LHCⅠ蛋白结构非常相似。 

Cloning and sequence analysis of the light harvesting complex Ⅰ gene（Lhca1） from three kinds of Phyllostachys and structure prediction of the protein

Light harvesting complexⅠ(LHCⅠ), is a chlorophyll a/bbinding protein, which plays a key role in plant photosynthesis. A full length cDNA encoding Lhca1 gene was cloned from the first strand of Phyllostachys edulis cDNA through RT PCR method, named as LhcaPe01 (GenBank EU035496). 616 bp cDNA fragment is cloned from the first strand of P. iridescens cDNA, named as LhcaH01 (GenBank EU513200) and 613 bp cDNA fragment is cloned from the first strand of P. fimbriligula cDNA, named as LhcaJ01 (GenBank EU513201). The length of LhcaPe01 is 1 051 bp，which contains an open reading frame encoding 249 amino acids from 39 to 779 positions and has 38 bp and 242 bp untranslated regions of 5′ end and 3′ end, respectively. There is a 30 bp Poly(A) in 3′ end. The pI and Mw of protein encoded by LhcaPe01 are predicted to be 5.400 0 and 22 107.34 D, respectively. The similarity of LhcaPe01 DNA sequence and encoding amino acid sequence blast with barley is the highest in Gramineae family. In Phyllostachys genus, the Lhca1 similarity of P. iridescens, P. fimbriligula and P. edulis is more higher than that in Gramineae family. The prediction of the protein structure indicates that Moso bamboo, barley, rice and maize have high similarity.