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| 卡片PCR法检测果汁饮料中卡耶他环孢子虫技术的建立 | |
| 引用本文： | 黄燕,张龙现,李晓虹,徐梅倩,朱顺海,曹杰,何国声.卡片PCR法检测果汁饮料中卡耶他环孢子虫技术的建立[J].中国兽医寄生虫病,2010,18(1):59-63. |
| 作者姓名： | 黄燕 张龙现 李晓虹 徐梅倩 朱顺海 曹杰 何国声 |
| 作者单位： | 1. 中国农业科学院上海兽医研究所,农业部动物寄生虫学重点开放实验室,上海,200241 2. 河南农业大学牧医工程学院,郑州,450002 3. 上海出入境检验检疫局,上海,200336 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项，河南省高校人才培养工程项目，上海市科学技术委员会标准专项基金 |
| 摘 要： | 为建立一种快速检测果汁饮料中卡耶他环孢子虫的方法，采用FTA卡片法提取果汁饮料中的卡耶他环孢子虫DNA，利用特异性引物组F1E／R2B、CC719／CRP999进行巢式PCR。以柔嫩艾美耳球虫、贝氏隐孢子虫等作为对照，结果显示，除卡耶他环孢子虫外，均无特异性条带。用卡耶他环孢子虫卵囊进行巢式PCR敏感性试验，结果显示敏感度最高达1．0×10^0个／mL。可见，FTA．PCR方特异、敏感，能用于新鲜食品中卡耶他环孢子虫的检测。 |
| 关 键 词： | 卡耶他环孢子虫 巢式PCR FTA |
FTA-PCR METHOD FOR DETECTION OF CYCLOSPORA CAYETANENSIS IN JUICE |
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| HUANG Yan,ZHANG Long-xian,LI Xiao-hong,XU Mei-qian,ZHU Shun-hai,CAO Jie,HE Guo-sheng.FTA-PCR METHOD FOR DETECTION OF CYCLOSPORA CAYETANENSIS IN JUICE[J].Chinese Journal of Veterinary Parasitology,2010,18(1):59-63. | |
| Authors: | HUANG Yan ZHANG Long-xian LI Xiao-hong XU Mei-qian ZHU Shun-hai CAO Jie HE Guo-sheng |
| Institution: | HUANG Yan~1,ZHANG Long-xian~2,LI Xiao-hong~3,XU Mei-qian~1,ZHU Shun-hai~1,CAO Jie~1,HE Guo-sheng~1 (1.Key Laboratory of Animal Parasitology of Ministry of Agriculture,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China,2.Engineering College of Animal Science , Veterinary Medicine,He\' nan Agricultural University,Zhengzhou 450002,3.Shanghai Entry-Exit Inspection , Quarantine Bureau,Shanghai 200336,China) |
| Abstract: | A 298-bp region of the small subunit rRNA gene was extracted from Cyclospora cayetanensis in juice by FTA and amplified in a nested PCR using unique primers ofF 1E/R2B and CC719/CRP999. The amplified product was specific for C. cayetanensis. No DNA amplification occurred when DNA templates from Eimeria tenella and C. baileyi were used. The sensitivity was 1.0×10^2 oocyst/mL. The FTA-PCR assay appears to be specific and sensitive and can be used for the detection of C. cayetanensis in fresh food. |
| Keywords: | FTA |
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