七子花愈伤组织诱导初探

对七子花愈伤组织的培养途径作了初步的探索,研究了以七子花的越冬芽及嫩叶诱导的愈伤组织、无菌短枝扦插和叶柄培养3条组织培养的途径来进行的七子花种质资源保护.结果表明:作为外植体,栽培的七子花叶片较野生的七子花越冬芽好;以MS+2,4-D2+ZT2培养基最利于幼叶的愈伤组织诱导;短枝无菌扦插培养的存活率高于叶柄培养.比较和分析了各条途径的优点及存在的问题,并对七子花的组织培养作了新的展望.

Preliminary studies on callus inducement of Heptacodium miconioides Rehd

The avenues of the callus tissue culture of \%Heptacodium miconioides\% Rehd. were preliminarily explored in this paper. Three relevant ways which could be used in the protection of the germplasm resource of \%Heptacodium miconioides\% Rehd.were studied (inducing callus with overwintering buds and tender leaves, bacteria\|free brachyblast cutting and leafstalk culture). The result showed that tender leaves of cultured \%Heptacodium miconioides\% were better than overwintering buds of wild \%Heptacodium miconioides\% as explants of callus;MS+2,4\|D2+ZT2 culture medium was the most beneficial medium to callus inducement of tender leaves; survival rate of bacteria\|free brachyblast cutting culture was higher than that of leafstalk culture.The advantages and disadvantages of each way were analyzed. This study showed the great potentiality of the tissue culture of \%Heptacodium miconioides\% Rehd.