| 应用H—Y单克隆抗体鉴定小鼠和家兔胚胎性别 |
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| 引用本文： | 曹文广,董伟.应用H—Y单克隆抗体鉴定小鼠和家兔胚胎性别[J].畜牧兽医学报,1993,24(6):506-511. |
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| 作者姓名： | 曹文广 董伟 |
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| 作者单位： | 北京农业大学
(曹文广，董伟)，北京农业大学(毛尧伴) |
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| 摘 要： | 以Ｃ５７ＢＬ／６雄性小鼠的脾细胞免疫同雌性小鼠，选择免疫应答反应较好的雌性小鼠４只，取其脾细胞与ＳＰ２／０骨髓瘤细胞进行融合的杂交瘤细胞经过３次克隆，筛选出了２株能够分泌Ｈ－Ｙ抗体的杂交瘤细胞系ＨＹＡ１和ＨＹＡ２，其分泌的相应抗体分别名为ＨＹａ１和ＨＹａ２。琼脂双扩散试验表明，ＨＹａ１和ＨＹａ２分别属于ＩｇＭ和ＩｇＧ亚类。胚间接免疫荧炮分析及胚胎细胞毒试验表明，ＨＹａ１和ＨＹａ２分别属于ＩｇＭ和Ｉ
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| 关 键 词： | 单克隆抗体 胚胎性别 鉴定 小鼠 |
IDENTIFICATION OF THE SEXES OF MOUSE AND RABBIT EMBRYOS BY H-Y MONOCLONAL ANTIBODIES |
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| Cao Wenguang,Dong Wei,Mao Yiaoban.IDENTIFICATION OF THE SEXES OF MOUSE AND RABBIT EMBRYOS BY H-Y MONOCLONAL ANTIBODIES[J].Acta Veterinaria et Zootechnica Sinica,1993,24(6):506-511. |
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| Authors: | Cao Wenguang Dong Wei Mao Yiaoban |
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| Institution: | Beijing Agricultural University |
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| Abstract: | H-Y monoclonal antibodies, IgM and IgG subclasses were produced, and used to determine sex of 8-cell to morula stage mouse and rabbit embryos by indirect immuno-fluorescence assay. A total of 438 mouse embryos were treated, 219 (50.0%) and 207 (47.3%) displayed specific fluorecence (H-Y positive) and non-specific fluorescence (H-Y negtive) respectively, except 12 (2.7%) unidentifed. Of 86 rabbit embryos, 42 (48.8%), 39 (45.3%) and 5(5.8%) were H-Y positive, H-Y negtive and unidentified respectively. 18 (81.8%) male pups and 4 (18.2%) femal pups were born following transfer of 199 mouse embryos with specific fluoresence, while 17(89.5%) female and 2 (10.5%) male pups were resulted from 183 mouse embryos with nonspecific fluorecence. |
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| Keywords: | H-Y antigen Monoclonal antibodies Embryo sexing |
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