微波消解-电感耦合等离子体质谱法测定怀牛膝中重金属元素含量

[目的]建立微波消解-电感耦合等离子体质谱(ICP-MS)的方法,测定怀牛膝中铅、镉、汞、砷含量。[方法]优化ICP-MS的操作条件,将样品进行微波消解,以114In作为内标,补偿基体效应,采用ICP-MS法同时测定上述4种元素。[结果]对于所测元素,标准曲线的相关系数r>0.9995,回收率为96.7%~106.4%,RSD<11.2%;样品测定结果表明,供试怀牛膝药材重金属超标现象较为严重。[结论]该研究建立的微波消解-ICP-MS法简便、快速、准确、灵敏度高,可为不同产地不同种类的中药材提供重金属含量的测定方法,用于中药材的质量控制。

Determination on Heavy Metals Content of Achyranthes bidentata Blume through Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

[Objective] The inductively coupled plasma mass spectrometry(ICP-MS) was constructed to determine the contents of Lead,Cadmium,Mercury and Arsenic in Archyranthes bidentata Blume.[Method] Through the optimum operation of ICP-MS,the samples were digested by microwave.The element 114 In was taken as an internal standard element to compensate body effect and ICP-MS method was used to determine the contents of Lead,Cadmium,Mercury and Arsenic.[Result] For the determined elements,the correlation coefficient(r) of standard curve was over 0.999 5 and recovery rate was from 96.7% to 106.4% while RSD was less than 11.2%.The result of determination showed that the heavy metal content in Archyranthes bidentata Blume beyond standard was serious.[Conclusion] The constructed ICPMS method with simple operation,rapid response,accuracy and high sensitivity in this experiment could be used for quality control of Chinese Medicinal Materials by detecting heavy metal contents in different Chinese Medicinal Materials from original places.