| 匐匍翦股颖L-93抗旱变异体的离体筛选与鉴定 |
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| 引用本文： | 周小梅,赵运林,周朴华,董萌,孙玉珍,杨小琴.匐匍翦股颖L-93抗旱变异体的离体筛选与鉴定[J].草业学报,2007,16(5):121-126. |
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| 作者姓名： | 周小梅 赵运林 周朴华 董萌 孙玉珍 杨小琴 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南,长沙,410128;湖南城市学院,湖南,益阳,413000;湖南城市学院,湖南,益阳,413000;湖南农业大学生物科学技术学院,湖南,长沙,410128 |
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| 摘 要： | 以匐匍翦股颖L-93的胚性愈伤为试验材料,用适宜剂量为20 Gy的60CoY射线进行辐射处理,将经辐照处理的胚性愈伤接种在含有PEG6000浓度为200 g/L的NB筛选培养基中进行抗旱筛选,获得耐旱再生植株,并对这些植株进行形态学以及干旱胁迫后植物的游离脯氨酸(Pro)的积累、叶绿素含量、细胞膜相对透性以及过氧化物酶(POD)、超氧物歧化酶(SOD)、过氧化氢酶(CAT)活性等方面进行抗旱性鉴定。结果表明,变异体植株的抗旱性明显强于对照。
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| 关 键 词： | 匐匍翦股颖 L-93 抗旱变异体 离体筛选 鉴定 |
| 文章编号： | 1004-5759(2007)05-0121-06 |
| 收稿时间： | 2006-08-06 |
| 修稿时间： | 2006骞?鏈?鏃?/revised_time> |
In vitro selection and identification of drought-resistant variants of Agrostis stolonifera L-93 |
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| ZHOU Xiao-mei,ZHAO Yun-lin,ZHOU Pu-hua,DONG Meng,SHUN Yu-zhen,YANG Xiao-qin.In vitro selection and identification of drought-resistant variants of Agrostis stolonifera L-93[J].Acta Prataculturae Sinica,2007,16(5):121-126. |
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| Authors: | ZHOU Xiao-mei ZHAO Yun-lin ZHOU Pu-hua DONG Meng SHUN Yu-zhen YANG Xiao-qin |
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| Institution: | 1. Bioscience and Technology College of Hunan Agriculture University, Changsha 410128, China; 2. Hunan City University, Yiyang 413000, China |
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| Abstract: | Embryogenic callus of Agrostis stolonifera L-93 was treated with 20 Gy 60Co gamma-ray and cultured in NB selective medium containing 200 g/L of PEG6000 and drought-resistant regenerated plants were obtained.Such plants were identified on morphology and on drought-tolerance from the Pro accumulation,chlorophyll content,cell membrane relative penetrability,peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT) activity after drought-stress.The results indicated that the drought-tolerance of variants was higher than that of controls. |
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| Keywords: | Agrostis stolonifera L-93 drought-resistant variants In vitro selection identification |
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