| 犬布氏杆菌Cu/Zn-SOD基因的原核表达及多克隆抗体的制备 |
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| 引用本文： | 曾宪斌,熊永忠,贾坤,张敦伟,佘利旋,夏惠焰,远立国,李守军.犬布氏杆菌Cu/Zn-SOD基因的原核表达及多克隆抗体的制备[J].中国预防兽医学报,2011,33(10):820-823. |
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| 作者姓名： | 曾宪斌 熊永忠 贾坤 张敦伟 佘利旋 夏惠焰 远立国 李守军 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东广州,510642
2. 中国农业科学院哈尔滨兽医研究所哈尔滨维科生物技术开发公司,黑龙江哈尔滨,150069 |
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| 基金项目： | 广东省科技计划项目(2008A060202017、2009B060300024) |
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| 摘 要： | 为检测犬布氏杆菌(Brucella canis)重组Cu/Zn-SOD蛋白的抗原性,本研究通过PCR方法扩增B.canis的Cu/Zn-SOD基因,将其克隆于表达载体pET-28a(+)中,构建重组表达质粒pET-SOD.将该质粒转化受体菌E.coli BL21 (DE3),经IPTG诱导后,SDS-PAGE电泳分析,...
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| 关 键 词： | 犬布氏杆菌 Cu/Zn-SOD 原核表达 抗原性 多克隆抗体 |
Prokaryotic expression of Brucella canis Cu/Zn-SOD gene and preparation of polyclonal antibody |
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| ZENG Xian-bin,XIONG Yong-zhong,JIA Kun,ZHANG Dun-wei,SHE Li-xuan,XIA Hui-yan,YUAN Li-guo,LI Shou-jun.Prokaryotic expression of Brucella canis Cu/Zn-SOD gene and preparation of polyclonal antibody[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(10):820-823. |
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| Authors: | ZENG Xian-bin XIONG Yong-zhong JIA Kun ZHANG Dun-wei SHE Li-xuan XIA Hui-yan YUAN Li-guo LI Shou-jun |
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| Institution: | ZENG Xian-bin 1,XIONG Yong-zhong 2,JIA Kun 1,ZHANG Dun-wei 1,SHE Li-xuan 1,XIA Hui-yan 1,YUAN Li-guo 1,LI Shou-jun 1 (1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2.Harbin Weike Biotechnology Development Company,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Science,Harbin 150069,China) |
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| Abstract: | To evaluate the immunogenicity of Brucella canis recombinant Cu/Zn-SOD protein,the Cu/Zn-SOD gene was amplified by PCR and expressed in E.coli BL21(DE3).The recombinant Cu/Zn-SOD protein was about 20 ku and in soluble form analyzed by SDS-PAGE,Western blot analysis showed that the recombinant protein reacted with positive sera of B.canis.Furthermore,the antiserum was prepared in rabbits by immunization of the purified recombinant protein.Western blot analysis demonstrated that the antiserum showed specific ... |
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| Keywords: | Brucella canis Cu/Zn-SOD prokaryotic expression antigenicity polyclonal antibody |
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