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利用SSR鉴定水稻杂交种子纯度的研究
引用本文:苏顺宗,黄玉碧,杨俊品,丁仲芳,高世斌.利用SSR鉴定水稻杂交种子纯度的研究[J].种子,2003(1):23-25.
作者姓名:苏顺宗  黄玉碧  杨俊品  丁仲芳  高世斌
作者单位:1. 四川农业大学玉米研究所,四川,雅安,625014
2. 四川省农业科学院作物研究所,成都,610066
3. 四川省成都市种子站,成都,610041
摘    要:为发展DNA标记技术在种子纯度鉴定上的应用,对12个目前在生产上大面积推广的水稻杂交种进行了SSR纯度鉴定。筛选出一批在常用亲本上具有较高多态性的SSR引物,并与田间纯度鉴定结果进行比较研究。结果表明:SSR鉴定的平均纯度与田间鉴定结果无显著差异,SSR可以用于杂交水稻种子纯度鉴定。

关 键 词:杂交种子  水稻  SSR  种子纯度  纯度鉴定

The Study on Testing the Seeds Purity of Hybrid Rice by SSR
Su Shunzong ,Huang Yubi ,Yang Junpin ,Ding Zhongfang ,Gao Shibin.The Study on Testing the Seeds Purity of Hybrid Rice by SSR[J].Seed,2003(1):23-25.
Authors:Su Shunzong  Huang Yubi  Yang Junpin  Ding Zhongfang  Gao Shibin
Institution:Su Shunzong 1,Huang Yubi 1,Yang Junpin 2,Ding Zhongfang 3,Gao Shibin 1
Abstract:In order to develop the application of DNA fingerprinting technique on testing the seeds Purity,Twelve varieties of hybrid rice popularized widely in china were chosen to test their seeds purity by SSR. Some high polymorphic SSR primers were screened between the parents of hybrid rice, and the results of seeds purity by SSR testing and by morphological identification. The results show that no significant difference was found between the two methods, so the SSR technique can be applied to identify the seeds purity of hybrid rice.
Keywords:Rice  SSR  Seeds purity  
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