花分生组织决定基因APETALA1转化油菜

拟南芥的APETALA1(AP1)是花分生组织的决定基因,同时也是萼片和花瓣正常发育的控制因子.本研究构建了含有AP1基因的植物表达载体PCAP,启动子为CaMV 35S,通过农杆菌侵染法转化油菜.用PCR方法对转基因植株进行了DNA和RNA水平上的分子检测,证明AP1基因已经整合到转基因植株的基因组中,并且得到了有效的表达.获得的转基因植株也表现出提前开花的特性.试验结果表明,AP1基因在促进成花方面的关键作用不具有种属特异性,利用基因工程可以快速高效地进行油菜早熟性的遗传改良.

Transformation of Floral Meristem Identity Gene APETALA1 into Brassica napus

The APETALA1(AP1) gene of Arabidopsis thaliana specifies flower meristem identity and is required for normal development of sepals and petals.Driven by the CaMV 35S promoter,plant expression vector PCAP constructed with AP1 was introduced into the genome of Brassica napus by Agrobacterium tumefaciens mediated transformation.The AP1 gene was proved by PCR analysis to have been integrated into genome of transgenic Brassica napus and expressed effectively.The T0 generation of the transgenic plants could flower earlier than the non-transformed plants of Brassica napus.These results showed the crucial function of AP1 in promoting flowering was not species-specific.The genetic improvement of Brassica napus for early maturing could be achieved by genetic engineering effectively.