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| 三叶崖爬藤ISSR反应体系的初探 | |
| 引用本文： | 杨华,陈磊,宋绪忠. 三叶崖爬藤ISSR反应体系的初探[J]. 江苏林业科技, 2010, 37(4): 9-11 |
| 作者姓名： | 杨华 陈磊 宋绪忠 |
| 作者单位： | 1. 浙江省林业科学研究院,浙江,杭州,310023 2. 浙江科技学院,浙江,杭州,310023 |
| 基金项目： | 浙江省科学技术厅青年人才项目 |
| 摘 要： | 对三叶崖爬藤ISSR-PCR扩增反应体系的建立和优化进行探讨。结果表明,最适宜的PCR反应条件为20μLPCR反应体积中含有1×缓冲液,20 ng模板DNA,3.0 mmol/L MgCl2,0.2 mmol/L dNTPs,1.5 U Taq DNA聚合酶,0.5μmol/L引物。扩增程序为：94℃预变性5 min;再35个循环：94℃60 s,52℃60 s和72℃105 s;最后72℃延伸10 min。 |
| 关 键 词： | 三叶崖爬藤 ISSR 优化 |
Optimization conditions of ISSR reaction system for Tetrastigma hemsleyanum Diels et.Gilg. |
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| YANG Hua,CHEN Lei,SONG Xu-zhong. Optimization conditions of ISSR reaction system for Tetrastigma hemsleyanum Diels et.Gilg.[J]. Journal of Jiangsu Forestry Science & Technology, 2010, 37(4): 9-11 | |
| Authors: | YANG Hua CHEN Lei SONG Xu-zhong |
| Affiliation: | 1.Zhejiang Academy of Forestry,Hangzhou 310023,China;2.Zhejiang University of Science and Technology,Hangzhou 31023,China) |
| Abstract: | Factors which affect the ISSR analysis in the molecular study of Tetrastigma hemsleyanum were studied for optimizing conditions.The results showed that the conditions being suitable for ISSR-PCR of T.hemsleyanum were as follows： 1 buffer,20 ng template DNA,3.0 mmol/L MgCl2,0.2 mmol/L dNTPs,1.5U Taq DNA polymerase,0.5 μmol/L primer.The amplification cycling was 1 cycle at 94 ℃ for 5 min;35 cycles at 94 ℃ for 60 sec,52 ℃ for 60 sec,and 72 ℃ for 105 s,respectively,and 1 cycle at 72 ℃ for 10 min. |
| Keywords: | ISSR |
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