| 槟榔叶片总蛋白质提取及双向电泳条件初探 |
| |
| 引用本文： | 李涛,曾莉娟,王健华,刘志昕.槟榔叶片总蛋白质提取及双向电泳条件初探[J].热带作物学报,2009,30(6):746-750. |
| |
| 作者姓名： | 李涛 曾莉娟 王健华 刘志昕 |
| |
| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;中国热带农业科学院科技信息研究所,海南儋州,573717 |
| |
| 基金项目： | 国家科技支撑计划课题"热带农林重要有害生物检测监测预警技术研究" |
| |
| 摘 要： | 采用3种提取植物组织蛋白的方法(TCA/丙酮沉淀法、E-TCA法、酚法)提取槟榔叶片总蛋白,在蛋白产量,一维和二维电泳图谱等方面对3种方法的提取效果进行比较.结果表明,TCA/丙酮沉淀法效果较好,是槟榔叶片总蛋白提取的可选方法,同时优化了双向电泳的条件和上样量.
|
| 关 键 词： | 槟榔 蛋白提取 二维电泳 |
Establishment of Methods for the Extraction and Two-Dimensional Electrophoresis Analysis of Leaf Proteins of Areca catechu |
| |
| Li Tao,Zeng Lijuan,Wang Jianhua and Liu Zhixin.Establishment of Methods for the Extraction and Two-Dimensional Electrophoresis Analysis of Leaf Proteins of Areca catechu[J].Chinese Journal of Tropical Crops,2009,30(6):746-750. |
| |
| Authors: | Li Tao Zeng Lijuan Wang Jianhua and Liu Zhixin |
| |
| Institution: | Li Tao1,Zeng Lijuan1,2,Wang Jianhua1,Liu Zhixin1 1 Institute of Tropical Bioscience , biotechnology,CATAS,Ministry of Agriculture Key Laboratory for Tropical Crop Biotechnology,Haikou,Hainan 571710,2 Institute of Scientific , Technological Information,Danzhou,Hainan,573717 |
| |
| Abstract: | Proteins from betel nut (Areca catechu Linn.)were extracted by using three methods trichloroacetic acid (TCA) /acetone precipitation, trichloroacetic acid precipitation (E -TCA), and phenol extraction], and their productivity and profiles were assessed by means of SDS-PAGE and two-dimensional electrophoresis. Of these 3 methods the TCA/acetone precipitation method was found most effective for the extraction and two -dimensional electrophoresis of proteins from Areca leaves. The conditions of two - dimensio... |
| |
| Keywords: | Areca catechu Linn protein extraction two-dimensional electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
