香蕉多酚氧化酶基因编码序列的电子克隆 和生物信息学分析

用电子克隆方法获得香蕉多酚氧化酶（polyphenol oxidase，PPO）基因编码序列。采用生物信息学方法，对此序列编码的蛋白质从氨基酸组成、理化性质尧疏水性/亲水性、亚细胞定位、高级结构及功能域等方面进行了预测和分析。结果表明：香蕉PPO 基因编码序列全长1602bp，包含1 602 bp开放阅读框（open reading frame, ORF），含有PPO功能域和酪氨酸酶功能域，在序列组成、高级结构及活性位点等方面，与小麦等其他植物的PPO 基因具有高度的相似性。

In silico cloning and bioinformatics analysis of polyphenol oxidase gene coding sequence of banana

The banana polyphenol oxidase (PPO) gene coding sequence (CDS) was cloned in silico based on the EST sequences from Unigene of NCBI. Some characters of the protein encoded by PPO gene CDS were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequence, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein and function. Results showed that the coding sequence of banana PPO was 1 602 bp, containing a 1 602 bp ORF which encodes 533 AA. Banana PPO CDS contains two typical PPO domain and one PPO KFDV tyrosinase domain. It was highly conservative compared with PPO from several different plant species in sequence compositon, advanced structure and activity sites.