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| UK114基因的克隆和表达载体的构建 | |
| 引用本文： | 常泓,南庆贤,岳文斌.UK114基因的克隆和表达载体的构建[J].山西农业大学学报(自然科学版),2006,26(1):1-3. |
| 作者姓名： | 常泓 南庆贤 岳文斌 |
| 作者单位： | 1. 山西农业大学,生命科学学院,山西,太谷,030801 2. 中国农业大学,食品学院,北京,100083 3. 山西农业大学,动物科技学院,山西,太谷,030801 |
| 基金项目： | 国家自然科学基金资助项目(30271003)，山西省科技攻关项目(011030)，山西省青年基金资助项目(20021038)，山西农业大学博士基金项目，山西农业大学博士后基金项目 |
| 摘 要： | 本研究采用PCR法扩增得到重组UK114 cDNA序列,将其克隆于T-easy载体并进行了序列测定与分析。结果表明该序列全长1 017 bp,有一个开放的阅读框(40 nt~450 nt),可编码137个氨基酸(14.2 kDa),与UK114的序列比较,同源性为91%,突变的86个核苷酸中都为无义突变,开放阅读框中仅有一个核苷酸突变。此外,本实验构建了pBV114表达载体,选择阳性克隆提取质粒进行酶切,琼脂糖检测获得约1 kb和3.7 kb的两个片段。 |
| 关 键 词： | 钙激活酶激活因子 UK114 克隆 表达载体 |
| 文章编号： | 1671-8151(2006)01-0001-03 |
| 修稿时间： | 2005年11月4日 |
Progress in Studies of Calpain Activator and Clonging of UK114 and Constructing of its Expression Vector |
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| CHANG hong,NAN Qing-xian,YUE Wen-bin.Progress in Studies of Calpain Activator and Clonging of UK114 and Constructing of its Expression Vector[J].Journal of Shanxi Agricultural University,2006,26(1):1-3. | |
| Authors: | CHANG hong NAN Qing-xian YUE Wen-bin |
| Abstract: | By PCR method the recombinant cDNA of UK114 was obtained. After linked to pGM-T easy vector the product was sequenced,the result showed that the cDNA consisted of 1 017 bp,and its coding region was about 411 bp(40 nt~450 nt),which could encode 137 amino acids(14.2 kDa).Compared this product with UK114 that Colombo reported,the homologous was 91%.There were 86 mutation nucleotides,and they were all meaningless.There was only one mutation nucleotide in coding region.We attained the plasmid of pBV114 that was the expression vector of calpain activator cDNA.By using 0.8% agarose gel electrophoresis there were two bands appeared,one was 1kb,and the other was 3.7 kb. |
| Keywords: | Calpain activator UK114 Cloning Expression vector |
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