| 一株木薯生淀粉糖化酶菌株的分离及酶学性质研究 |
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| 引用本文： | 钟坤,谭德冠,孙雪飘,彭明,张家明.一株木薯生淀粉糖化酶菌株的分离及酶学性质研究[J].热带作物学报,2012,33(7):1239-1244. |
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| 作者姓名： | 钟坤 谭德冠 孙雪飘 彭明 张家明 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室 海南省热带生物质能源工程技术研究中心 海南海口 571101 |
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| 基金项目： | 科技部国际科技合作计划项目“热带生物质能源高效转化及综合利用”（No. 2010DFA62040）。 |
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| 摘 要： | 从腐烂的木薯中分离得到1株降解生淀粉能力较强的菌株ITBB FC2，经形态学鉴定和18S rDNA序列分析，初步鉴定属于黑曲霉（Aspergillus niger）。该菌株用2％生木薯粉培养基摇瓶，37 ℃，200 r/min培养3 d后，发酵液葡萄糖含量为970 mg/L，生淀粉酶活力为495.04 U/mL。采用固体产酶培养基（麸皮 ∶ 水=1 ∶ 1）培养，所得酶活力为6 330 U/g。酶反应最适pH为4.0~5.0。Ca2+对酶活力有一定的促进作用。
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| 关 键 词： | 黑曲霉 生淀粉糖化酶 酶学性质 |
Isolation of a RSDG-producing Strain Aspergillus niger ITBB FC2 and Its Enzymatic Characterization |
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| ZHONG Kun,TAN Deguan,SUN Xuepiao,PENG Ming and ZHANG Jiaming.Isolation of a RSDG-producing Strain Aspergillus niger ITBB FC2 and Its Enzymatic Characterization[J].Chinese Journal of Tropical Crops,2012,33(7):1239-1244. |
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| Authors: | ZHONG Kun TAN Deguan SUN Xuepiao PENG Ming and ZHANG Jiaming |
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| Institution: | Institute of Tropical Bioscience and Biotechnology/MOA Key Laboratory of Tropical Crop Biology and Genetic Resources/Hainnan Tropical Bioenergy Enginering & Technology Research Center,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China |
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| Abstract: | Aspergillus niger strain ITBB FC2,isolated from decayed raw cassava root showed strong raw-starchdigrading-glucoamylase(RSDG) activity in liquid fermentation medium with 2% cassava flour.The crude enzyme had an activity of 495.04 U/mL and produced 970 mg/L of glucose in the 2% cassava flour fermentation medium when inculated at 37 ℃,200 r/min for 3 days,while it had an activity of 6 330 U/g in solid fermentation medium(bran ∶ water=1 ∶ 1).The optimal pH was 4~5.2 mmol/L.Ca2+ promoted the enzyme activity by 133.74%. |
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| Keywords: | Aspergillus niger Raw-starch-digrading-glucoamylase Enzymatic characterization |
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