辣椒采后软腐病拮抗细菌的分离及生防效果

辣椒；软腐病；拮抗细菌；生防效果

Isolation and Biocontrol Efficacy of Antagonistic Bacteria against Bacterial Soft Rot of Pepper after Harvest

For the purpose of reducing pepper postharvest losing caused by bacterial soft rot,this study was carried out to isolate and screen antagonistic bacteria from pepper plants.The result showed that 3 strains of antagonist which could effectively control the bacterial soft rot were isolated,among them the strain PP-32 showed the most effective antagonizing activity,and its maximal inhibition circle diameter was 37 mm after incubated on the medium plate for 3 d,while the inhibition rate to the pathogeny was 56.7% after incubated for 7 d,and the strain PP-32 which had the characteristic of wide-spectrum antagonism was identified as Bacillus sp.preliminarily.The suspension(1.0 ×109cfu/mL)of the strain showed strong inhibiting effect to the growth of the pathogeny,however,its sterile cellular exudates showed no inhibiting effect.Further studies suggested that the strain had antagonizing effect 72.2% at least to the soft rot on pepper fruits and biocontrol efficacy 17.3% after the fruits stored at 25 ℃ for 18 d.As a result,the strain PP-32 could be applied as a biocontrol agent to control pepper postharvest bacterial soft rot.