桃金娘果实多酚抗氧化活性及乙酰胆碱酯酶抑制活性研究

为了探明桃金娘果实的多酚含量及其多酚的抗氧化活性和乙酰胆碱酯酶抑制活性，以为进一步开发利用奠定基础。以采自海南海口的桃金娘整果为实验材料，用95%体积分数的乙醇提取桃金娘多酚，采用福林酚法测定其总酚含量。对获得的桃金娘多酚进行DPPH、ABTS、羟基自由基清除率和乙酰胆碱酯酶抑制活性测定。结果表明，桃金娘的多酚含量较高，达2.45mg/g干物质，其对DPPH、ABTS和羟基自由基均具有显著的清除活性，并且其活性随着浓度的增加，清除能力也随之增强，其IC50分别为120.78、25.44、194.09μg/mL；桃金娘果实多酚对乙酰胆碱酯酶也显示出较强的抑制活性，IC50为15.07μg/mL，且随着浓度的增加，其抑制活性呈上升趋势。该实验显示桃金娘果实多酚不仅具有较好的抗氧化性还具有显著的乙酰胆碱酯酶抑制活性。

Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Rhodomyrtus tomentosa Fruit Polyphenol

The total phenolics were extracted from Rhodomyrtus tomentosa whole fruit with 95% methanol and quantified by Folin method. The extract was subjected to assessing its antioxidant potential using DPPH, ABTS and Hydroxyl system, and its acetylcholinesterase (AchE) inhibitory activity was tested by modified Edman method. The results showed that the fruit extract contained high levels of total phenolics (2.45 mg/g DW), and it was found to be effective scavenger against DPPH radical, ABTS and hydroxyl radical. The activities increased in a concentration dependent manner. IC50 values were 120.78, 25.44 and 194.09 μg/mL, respectively. It also exhibited strong inhibitory activity against acetylcholinesterase in a concentration dependent manner, IC50 was 15.07 μg/mL. In conclusion, the polyphenol extractives from R. tomentosa fruit had strong antioxidant potential and acetylcholinesterase inhibitory activity.