| 利用大白菜DH群体构建AFLP遗传连锁图谱 |
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| 引用本文： | 张晓芬,王晓武,娄平,张晓伟,王永清,原玉香,赵建军,孙日飞.利用大白菜DH群体构建AFLP遗传连锁图谱[J].园艺学报,2005,32(3):443-437. |
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| 作者姓名： | 张晓芬 王晓武 娄平 张晓伟 王永清 原玉香 赵建军 孙日飞 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所, 北京100081; 四川农业大学林学园艺学院, 雅安625014; 河南省农业科学院生物技术研究所, 郑州450002; 河北农业大学园艺学院, 保定071001 |
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| 基金项目： | 农业部蔬菜遗传与生理重点开放实验室资助项目 |
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| 摘 要： | 以大白菜‘汴早- 26’和‘光90E16’的F1 代进行游离小孢子培养所获得的含有59个株系的DH群体为试材, 利用AFLP技术通过63对引物筛选共获得346个AFLP多态性标记, 运用JoinMap 3.0软件构建大白菜遗传连锁图谱。该图谱主要包括10个连锁群, 总图距为708 cM, 平均图距为2.0 cM。
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| 关 键 词： | 大白菜 DH群体 AFLP 遗传连锁图谱 |
| 文章编号： | 0513-353X(2005)03-0443-06 |
| 收稿时间： | 2004-7-22 |
| 修稿时间： | 2004-10-9 |
An AFLP-based Genetic Linkage Map of Chinese Cabbage Using Double Haploid(DH) Population |
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| Zhang Xiaofen,Wang Xiaowu,LOU Ping,Zhang Xiaowei,Wang Yongqing,YUAN Yuxiang,ZHAO Jianjun,Sun Rifei.An AFLP-based Genetic Linkage Map of Chinese Cabbage Using Double Haploid(DH) Population[J].Acta Horticulturae Sinica,2005,32(3):443-437. |
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| Authors: | Zhang Xiaofen Wang Xiaowu LOU Ping Zhang Xiaowei Wang Yongqing YUAN Yuxiang ZHAO Jianjun Sun Rifei |
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| Institution: | (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Horticulture and Forestry, Sichuan Agricultural University, Yapan 625014, China; Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; College of Horticulture, Hebei Agricultural University, Baoding 071001, China) |
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| Abstract: | A genetic linkage map of Brassica rapa was constructed using AFLP markers,based on a F_1-derived doubled-haploid (DH) population with 59 lines.The map consists of 10 main linkage groups,including 346 AFLP markers generated by 63 primer combinations,and covers 708 cM with an average genetic distance of 2.0 cM. |
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| Keywords: | Chinese cabbage Double haploid AFLP Genetic linkage map |
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