| 小叶丁香的再生体系建立 |
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| 引用本文： | 武术杰.小叶丁香的再生体系建立[J].东北林业大学学报,2009,37(8). |
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| 作者姓名： | 武术杰 |
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| 作者单位： | 长春大学,长春,130022 |
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| 基金项目： | 吉林省教育厅科研项目 |
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| 摘 要： | 以小叶丁香的带花芽的茎段为外植体进行诱导培养,结果表明,茎段作为小叶丁香组织培养的外植体是适宜的.初代最佳培养基为MS+1.0mg/L 6-BA+0.1mg/L IBA,继代培养的最佳培养基为MS+1.0mg/L 6-BA+0.1mg/L IBA,生根培养最佳培养基为1/2MS+0.1mg/L NAA.培养条件为培养基中加入3%蔗糖、8g/L琼脂,pH=6.1.培养温度为24～26℃,光照时间为12～14h/d,光照强度为2200～2500lx.
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| 关 键 词： | 小叶丁香 组织培养 茎段 |
Regeneration System of Syringa microphylla |
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| Wu Shujie.Regeneration System of Syringa microphylla[J].Journal of Northeast Forestry University,2009,37(8). |
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| Authors: | Wu Shujie |
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| Institution: | Wu Shujie(School of Biological Science , Technology,Changchun University,Jilin 130022,P.R.China) |
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| Abstract: | Tissue culture of Syringa microphylla was conducted using stem segments with flower bud as explants.Result shows that the stem segments of S.microphylla are suitable to be used as explants.The optimal media for primary culture and successive culture of S.microphylla were both MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L IBA,and the optimal medium for rooting was 1/2 MS medium with 0.1 mg/L NAA.The optimum culture conditions were obtained as medium with 3 percent of sucrose and 8 g/L agar,pH 6.1,cu... |
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| Keywords: | Syringa microphylla Tissue culture Stem segments |
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