| 转基因抗虫棉品种(系)的遗传多样性初步研究 |
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| 引用本文： | 朱龙付,张献龙,聂以春,吴家和.转基因抗虫棉品种(系)的遗传多样性初步研究[J].华中农业大学学报,2002,21(5):401-405. |
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| 作者姓名： | 朱龙付 张献龙 聂以春 吴家和 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金重点项目 (3 983 0 2 40 ) |
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| 摘 要： | 利用简单重复序列（SSRs)和随机扩增多态性DNAs(RAPDs)2种分子标记对来自我国各个棉区的30份陆地棉转基因四种质材料进行了遗传多样性分析，材料包含有我国的3种转基因类型，利用NTSYS-pc2.10统计分析软件，采用Jaccard‘s相似系数UPGMA法进行聚类，结果表明，3类转基因材料大致分为不同的2类，而且不同棉区的转基因材料交叉分布，相似系数分析表明，我国转基因材料的遗传多样性相对较为丰富。同时对我国转基因材料的生产应用提供了参考意见。
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| 关 键 词： | 分子标记 SSRs RAPDs 转基因抗虫棉 品种 遗传多样性 |
| 修稿时间： | 2002年5月8日 |
Genetic Diversity among Transgenic Cotton Cultivars(lines) Analysed with RAPDs and SSRs |
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| Zhu Longfu,Zhang Xianlong,Nie Yichun,Wu Jiahe.Genetic Diversity among Transgenic Cotton Cultivars(lines) Analysed with RAPDs and SSRs[J].Journal of Huazhong Agricultural University,2002,21(5):401-405. |
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| Authors: | Zhu Longfu Zhang Xianlong Nie Yichun Wu Jiahe |
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| Abstract: | Thirty transgenic bollgard cotton ( Gossypium hirsutum L.) germplasms including 3 transgenic types collected from different planting areas in China were assessed by using simple sequence repeats(SSRs) and randomly amplified polymorphic DNAs(RAPDs). Jaccard's genetic similarity coefficients were calculated and dendrograms were constructed by the unweighted pair group method of arithmetic average (UPGMA) using the NTSYS-pc version 2.10. The result showed that all materials could be divided into two main groups, but were not correlated with their origins. The genetic similarity coefficients indicated that germplasms had relatively high level of genetic diversity. The results might be useful to guide the application of the transgenic cultivars. |
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| Keywords: | transgenic cotton genetic diversity SSRs RAPDs |
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