| 甘薯羽状斑驳病毒外壳蛋白基因的分子变异 |
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| 引用本文： | 王晓华,张振臣,乔奇,秦艳红,张德胜,田雨婷.甘薯羽状斑驳病毒外壳蛋白基因的分子变异[J].植物保护,2012,38(2):114-116. |
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| 作者姓名： | 王晓华 张振臣 乔奇 秦艳红 张德胜 田雨婷 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所,郑州,450002
2. 河南省农业科学院植物保护研究所,郑州450002;河南省农作物病虫害防治重点实验室,郑州450002 |
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| 基金项目： | 国家甘薯产业技术体系建设项目(nycytx-16-B-7) |
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| 摘 要： | 应用单链构象多态性(single-strand conformation polymorphism,SSCP)技术结合核苷酸序列测定的方法,对我国甘薯主产区11个省份的甘薯羽状斑驳病毒(Sweet potato feathery mottle virus,SPFMV)外壳蛋白(CP)基因的分子变异情况进行了研究.结果表明,SPFMV CP基因的RT-PCR产物表现了较丰富的图谱类型,50个分离物共产生9种主要的SSCP带型；对显示不同带型的20个样品的CP基因进行了序列测定和进化树分析,CP基因核苷酸序列一致性为77.2％～99.9％.说明这些样品的SPFMV的CP基因存在较大的分子变异,可划分为EA、RC、O和C4个株系.
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| 关 键 词： | 甘薯羽状斑驳病毒 外壳蛋白基因 分子变异 SSCP分析 |
Molecular variation of Sweet potato feathery mottle virus coat protein gene |
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| Wang Xiaohua
,
Zhang Zhenchen
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Qiao Qi
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Qin Yanhong
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Zhang Desheng
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Tian Yuting.Molecular variation of Sweet potato feathery mottle virus coat protein gene[J].Plant Protection,2012,38(2):114-116. |
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| Authors: | Wang Xiaohua Zhang Zhenchen Qiao Qi Qin Yanhong Zhang Desheng Tian Yuting |
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| Institution: | 1,2 (1.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Henan Key Laboratory of Crop Pest Control,Zhengzhou 450002,China) |
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| Abstract: | Virus samples were collected from 11 provinces in China,and the molecular variation of Sweet potato feathery mottle virus(SPFMV)coat protein(CP) gene were analyzed using single-strand conformation polymorphism(SSCP) and sequence analysis.SSCP analysis of 50 isolates showed that these isolates produced 9 kinds of SSCP band forms.The results of sequence comparison and phylogenetic analysis showed that the identities of the nucleotide sequences of CP genes among these isolates were 77.2%-99.9%.SPFMV CP genes of different isolates in China had great molecular variability and could be divided into four diverged groups at least,including the East Africa(EA),Russet Crack(RC),Ordinary(O) and Common(C) strain groups. |
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| Keywords: | SPFMV coat protein gene molecular variation SSCP analysis |
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