Effect of miR-7-5p targeting Itch gene on insulin resistance model of HepG2 cells |
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Authors: | WU Ya-liu MA Yu YU Man-li WANG Wen-dong HAO Min CHANG Xiao-tong |
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Institution: | Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 075000, China |
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Abstract: | AIM:To preliminarily explore the relationship between microRNA-7-5p (miR-7-5p) and Itch gene and their relationship with insulin resistance by establishing insulin resistance model of HepG2 cells in vitro for detecting differential expression of miR-7-5p and its predicted target gene Itch in the state of insulin resistance. METHODS:The insulin resistance model of HepG2 cells was induced by suitable concentration of plamitic acid. The possible target genes and the associated signaling pathways of miR-7-5p were predicted based on bioinformatic analysis. The expression levels of miR-7-5p and Itch were detected by RT-qPCR and Western blot in the HepG2 cells with insulin resistance. RESULTS:The HepG2 cell model of insulin resistance was successfully induced by treatment with 0.25 mmol/L palmitic acid for 24 h. Compared with negative control group, the expression level of miR-7-5p detected by RT-qPCR in insulin resistance group was significantly decreased (P<0.01). Bioinformatic analysis showed that a considerable number of target genes of miR-7-5p were enriched in the ubiquitin proteasome system. Among them, E3 ubiquitin ligase Itch gene was the most relevant target gene to insulin resistance. The results of Western blot showed that the protein expression of Itch was up-regulated in the HepG2 cells under insulin resistance (P<0.01). CONCLUSION:miR-7-5p may be involved in the pathophysiological process of insulin resistance, which may directly or indirectly affect the normal transduction of insulin signaling pathway by targeting Itch gene. |
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Keywords: | Insulin resistance HepG2 cells MicroRNA-7-5p Itch gene |
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