还原型谷胱甘肽对氟中毒小鼠附睾的影响

试验旨在研究还原型谷胱甘肽（reduced glutathione，GSH）对氟中毒小鼠附睾的影响。将50只3周龄ICR雄性小鼠随机分为5组，分别为对照组和4组染氟组。对照组饮用蒸馏水，染氟组分别供给含100 mg/L NaF的蒸馏水，自由饮水30 d。之后在染氟组中选择3组每日分别灌胃200、400和800 mg/（kg·只）GSH，同时各组小鼠再自由饮含氟水30 d。试验结束后，摘取小鼠附睾进行活性氧（ROS）、丙二醛（MDA）、GSH含量及谷胱甘肽过氧化物酶（GPx）、谷胱甘肽硫转移酶（GSTs）和谷胱甘肽还原酶（GR）活性检测，同时制作石蜡切片，HE染色后进行组织形态结构分析。结果显示，与对照组相比，100 mg/L NaF组精子密度、精子活力、GSH含量、GPx和GR活性均显著下降（P<0.05），而GSH添加组较100 mg/L NaF组均有所升高；100 mg/L NaF组精子畸形率、ROS和MDA含量较对照组均显著升高（P<0.05），而GSH添加组较100 mg/L NaF组均降低，其中800 mg/kg GSH组效果明显。与对照组相比，100 mg/L NaF组附睾头、附睾体及附睾尾管腔厚度升高，管腔内精子数量下降；GSH添加组附睾头、附睾体及附睾尾的管腔厚度与管腔内精子数量都有所恢复，其中800 mg/kg GSH组效果较好。综上所述，添加GSH后氟中毒小鼠谷胱甘肽抗氧化系统酶活性有所升高，附睾组织形态结构一定程度上有所恢复，对附睾有一定的改善作用。

Effects of Reduced Glutathione on the Epididymis of Mice with Fluorosis

To investigate the effect of reduced glutathione (GSH) on the epididymis of mice with fluorosis,50 ICR male mice were randomly divided into five groups:Control group and 4 fluorosis groups,which were given distilled water and distilled water +100 mg/L NaF for 30 d,respectively. Then 3 of 4 fluorosis groups were given 200,400 and 800 mg/kg GSH,respectively. After feeding for 30 d,the epididymis was collected and morphological structure were observed after HE staining,the levels of ROS,MDA and GSH,and the activities of GPx,GSTs and GR in the epididymis were detected. The results showed that compared with the control group,the sperm density,sperm motility,GSH,GPx and GR in 100 mg/L NaF group were all significantly decreased (P<0.05),while that in GSH addition groups were increased than 100 mg/L NaF group,and 800 mg/kg GSH group had the best effect. The rate of teratosperm, ROS and MDA were significantly elevated (P<0.05),while that all decreased in GSH addition groups. Compared with the control group,the epididymis luminal thickness in 100 mg/L NaF group were increased and the number of sperm in the lumen was decreased and the indexes had been restored after GSH supplement,and 800 mg/kg GSH group had the better effect.In summary,after adding GSH,the activity of enzyme in glutathione antioxidant system of mice with fluorosis was increased, epididymis morphology had been restored to a certain extent.