三穗麻鸭SOCS1基因克隆及序列分析

为了分析三穗麻鸭细胞因子信号传导抑制蛋白（suppressors of cytokine signaling，SOCS）基因分子特征，预测其编码蛋白的生物学功能，本试验对三穗麻鸭SOCS1基因进行PCR扩增、克隆及序列测定，应用生物信息学方法对三穗麻鸭SOCS1基因进行序列分析，并对其编码蛋白的二级结构、保守结构域、跨膜结构域、信号肽和三级结构进行预测。结果显示，三穗麻鸭SOCS1基因全长为624 bp，可编码207个氨基酸；与大雁、原鸡、非洲爪蟾、猪、牛、人、大鼠和草鱼相应序列核苷酸序列同源性分别为97.6%、92.6%、68.3%、65.0%、64.8%、64.5%、63.5%和58.2%，氨基酸序列同源性分别为99.5%、96.6%、69.2%、64.0%、64.0%、67.9%、65.4%和50.8%；系统进化树显示，三穗麻鸭与大雁亲缘关系最近；编码蛋白的二级结构由无规则卷曲、α-螺旋、β-转角和延伸链组成，具有一个中央SH2区和SOCS盒，且无跨膜区和信号肽区域；三级结构呈弯曲螺旋结构。本试验结果为三穗麻鸭SOCS1基因编码蛋白的生物学功能研究奠定了理论基础。

Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck

In order to analyze the molecular characteristics of suppressors of cytokine signaling (SOCS) gene in Sansui duck,and predict the biological function of SOCS1 gene encoded protein,the SOCS1 gene was amplified,cloned and sequenced,the secondary structure,conserved domain analysis,transmembrane domain,signal peptide and tertiary structure of SOCS1 protein were predicted using bioinformatic methods.The results showed that the length of SOCS1 gene was 624 bp,encoding 207 amino acids.The SOCS1 gene of Sansui duck was shared a nucleotide identity of 97.6%,92.6%,68.3%,65.0%,64.8%,64.5%,63.5% and 58.2%,and an amino acid identity of 99.5%,96.6%,69.2%,64.0%,64.0%,67.9%,65.4% and 50.8% with those of Anser cygnoides,Gallus gallus,Xenopus laevis,Sus scrofa,Bos taurus,Homo sapiens,Rattus norvegicus and Ctenopharyngodon idella,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between Sansui duck and Anser cygnoides.The secondary structure of the encoding protein was based with random coil,alpha helix,beta turn and extended strand region,with a central SH2 domain and a SOCS box.There was no the transmembrane domains and the signal peptide,and with the curved spiral tertiary structure.These results provided a theoretical basis for the study of the biological function of SOCS1 gene encoding protein in Sansui duck.